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Original Research (Original Article) 
JVS. 2020; 7(1): 77-85


process development of nano emulsion foot and mouth disease virus vaccine

SOAD EID ELSAYED, mohammed abdelhamid shalaby, Ausama abdelraouf Yousef , Abu bakr ahmed Agor , Sherouk Essam Aly.

Abstract
Background: Foot and mouth disease is a highly contagious disease of livestock that is endemic in Egypt with severe economic impact. The main method of control is through vaccination of the livestock with a potent vaccine.
Objectives: process development of the current formulations and generation new ones is crucial. Currently, Montanide ISA206 is the adjuvant used in FMDV vaccine produced in Egypt which is w/o/w microemulsion.
Methods: we aimed to increase the efficacy of the conventional formulation by converting the microemulsion to Bio-compatible and stable Nano emulsion. Emulsification was applied through ultrasonication of Montanide ISA 206 in the presence of Tween 80 as a surfactant. The physical characteristics were studied and imaged by TEM.
Results: Images showed particle size ranged from 200-400 nm instead of 1 micron of the original. Immunological evaluation by serum neutralization test showed enhanced immunogenicity of developed formula when compared to the conventional one. Challenge test revealed high protection levels that reached 90% of the Guinea pig vaccinated by nano emulsion.
Conclusion: our finding suggest that we aimed to enhance immunity through nano emulsion of Montanide ISA 206 is well achieved.

Key words: FMD , Nano emulsion , TEM , VNT


 
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How to Cite this Article
Pubmed Style

SEE, shalaby ma, AaY, AbaA, Aly SE, . process development of nano emulsion foot and mouth disease virus vaccine. JVS. 2020; 7(1): 77-85.


Web Style

SEE, shalaby ma, AaY, AbaA, Aly SE, . process development of nano emulsion foot and mouth disease virus vaccine. http://www.j-virol-sci.com/?mno=81029 [Access: December 22, 2020].


AMA (American Medical Association) Style

SEE, shalaby ma, AaY, AbaA, Aly SE, . process development of nano emulsion foot and mouth disease virus vaccine. JVS. 2020; 7(1): 77-85.



Vancouver/ICMJE Style

SEE, shalaby ma, AaY, AbaA, Aly SE, . process development of nano emulsion foot and mouth disease virus vaccine. JVS. (2020), [cited December 22, 2020]; 7(1): 77-85.



Harvard Style

, S. E. E., shalaby, m. a., , A. a. Y. ., , A. b. a. A. ., Aly, S. E. & (2020) process development of nano emulsion foot and mouth disease virus vaccine. JVS, 7 (1), 77-85.



Turabian Style

, SOAD EID ELSAYED, mohammed abdelhamid shalaby, Ausama abdelraouf Yousef, Abu bakr ahmed Agor, Sherouk Essam Aly, and . 2020. process development of nano emulsion foot and mouth disease virus vaccine. Journal of Virological Sciences, 7 (1), 77-85.



Chicago Style

, SOAD EID ELSAYED, mohammed abdelhamid shalaby, Ausama abdelraouf Yousef, Abu bakr ahmed Agor, Sherouk Essam Aly, and . "process development of nano emulsion foot and mouth disease virus vaccine." Journal of Virological Sciences 7 (2020), 77-85.



MLA (The Modern Language Association) Style

, SOAD EID ELSAYED, mohammed abdelhamid shalaby, Ausama abdelraouf Yousef, Abu bakr ahmed Agor, Sherouk Essam Aly, and . "process development of nano emulsion foot and mouth disease virus vaccine." Journal of Virological Sciences 7.1 (2020), 77-85. Print.



APA (American Psychological Association) Style

, S. E. E., shalaby, m. a., , A. a. Y. ., , A. b. a. A. ., Aly, S. E. & (2020) process development of nano emulsion foot and mouth disease virus vaccine. Journal of Virological Sciences, 7 (1), 77-85.








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