Open Access and Peer-reviewed
Home Journal Issues Guide for Authors Editorial Board Aims & Scope About Journal News & Announcements


Original Research (Original Article) 
JVS. 2020; 7(1): 66-76


Comparative Growth Kinetic of Egyptian LSDV isolate in Vero and MDBK cell line

Mohamed Mahmoud Mashaly, Ayman Hany El-Deeb , Momtaz Abdelhady Shahein, Hussein Ali Hussein.

Abstract
Background: Lumpy skin disease outbreaks were observed in different governorates of Egypt affecting cattle throughout the year and are still present. The progressive shift of the outbreak in Egypt during 2018 causing severe losses in cattle population despite vaccination, which may incur some debates on the efficacy of current live attenuated vaccines against CaPVs. The virus isolation, identification, and vaccine or other diagnostic preparations mainly depend on the proper culture used.
Objective: Our aim was to study the growth kinetic of recent LSDV isolate to determine the culture properties of recent LSDV isolated from skin nodule that could be useful in the attempts to develop more effective LSDV vaccine and diagnostic purposes.
Methods: In order to isolate the LSDV MDBK continuous cell line was used for six serial passages. The obtained LSDV isolate was molecularly identified and deposited to the GenBank accession number of MN271728. In addition, a comparative study of LSDV growth curves of two permissible continuous cell lines (MDBK and Vero). The growth curve of LSDV was determined by inoculating both cultures, harvesting at intervals up to 24 hours and titrating the virus culture. To confirm LSDV accumulated in cell cultures, molecular identification is required; we carried Real-time PCR.
Results: This study has revealed that the virus culture period until 95% CPE was 48hr for MDBK and 72hr for Vero culture. The significant difference in growth behavior could be found between its growth on MDBK and Vero cells that it reaches its highest peak at 48hr for MDBK and 72hr for Vero cells. The maximum destruction in cell monolayers was observed within 72hr to 96hr post-infection. With an optimal condition for both cultures, the LSDV titers were 5.2 in MDBK after 48hr and 5.67 in Vero after 72hr. This rise in virus titer was followed by a sharp decrease after 48hr P.I. on MDBK unlike Vero cells that continue its rise in virus titer until reaching its peak at 72hr P.I.
Conclusion: Comparison of growth kinetic of LSDV on MDBK and Vero cell line revealed that Vero cell was considered the best susceptible cell for the propagation of LSDV with best harvesting time 72hr P.I to obtain a maximum titer for subsequent vaccine production.

Key words: LSDV, isolation, MDBK, Vero, Growth kinetic, Cytopathic effect, Real-time PCR, Egypt.


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Mohamed Mahmoud Mashaly
Articles by Ayman Hany El-Deeb
Articles by Momtaz Abdelhady Shahein
Articles by Hussein Ali Hussein
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Mashaly MM, AHE, Shahein MA, Hussein HA, . Comparative Growth Kinetic of Egyptian LSDV isolate in Vero and MDBK cell line. JVS. 2020; 7(1): 66-76.


Web Style

Mashaly MM, AHE, Shahein MA, Hussein HA, . Comparative Growth Kinetic of Egyptian LSDV isolate in Vero and MDBK cell line. http://www.j-virol-sci.com/?mno=75364 [Access: December 22, 2020].


AMA (American Medical Association) Style

Mashaly MM, AHE, Shahein MA, Hussein HA, . Comparative Growth Kinetic of Egyptian LSDV isolate in Vero and MDBK cell line. JVS. 2020; 7(1): 66-76.



Vancouver/ICMJE Style

Mashaly MM, AHE, Shahein MA, Hussein HA, . Comparative Growth Kinetic of Egyptian LSDV isolate in Vero and MDBK cell line. JVS. (2020), [cited December 22, 2020]; 7(1): 66-76.



Harvard Style

Mashaly, M. M., , A. H. E. ., Shahein, M. A., Hussein, H. A. & (2020) Comparative Growth Kinetic of Egyptian LSDV isolate in Vero and MDBK cell line. JVS, 7 (1), 66-76.



Turabian Style

Mashaly, Mohamed Mahmoud, Ayman Hany El-Deeb, Momtaz Abdelhady Shahein, Hussein Ali Hussein, and . 2020. Comparative Growth Kinetic of Egyptian LSDV isolate in Vero and MDBK cell line. Journal of Virological Sciences, 7 (1), 66-76.



Chicago Style

Mashaly, Mohamed Mahmoud, Ayman Hany El-Deeb, Momtaz Abdelhady Shahein, Hussein Ali Hussein, and . "Comparative Growth Kinetic of Egyptian LSDV isolate in Vero and MDBK cell line." Journal of Virological Sciences 7 (2020), 66-76.



MLA (The Modern Language Association) Style

Mashaly, Mohamed Mahmoud, Ayman Hany El-Deeb, Momtaz Abdelhady Shahein, Hussein Ali Hussein, and . "Comparative Growth Kinetic of Egyptian LSDV isolate in Vero and MDBK cell line." Journal of Virological Sciences 7.1 (2020), 66-76. Print.



APA (American Psychological Association) Style

Mashaly, M. M., , A. H. E. ., Shahein, M. A., Hussein, H. A. & (2020) Comparative Growth Kinetic of Egyptian LSDV isolate in Vero and MDBK cell line. Journal of Virological Sciences, 7 (1), 66-76.








AUTHOR LOGIN

REVIEWER LOGIN

Indexed
&
Abstracted



ABOUT JOURNAL
POLICIES
STATEMENTS

This is an open access journal which means that all content is freely available without charge to the user or his/her institution. Users are allowed to read, download, copy, distribute, print, search, or link to the full texts of the articles in this journal without asking prior permission from the publisher or the author. This is in accordance with the Budapest Open Access Initiative (BOAI) definition of open access.

The articles in Journal of Virological Sciences are open access articles licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc-sa/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.