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Original Research (Original Article) 
JVS. 2020; 7(1): 19-31


In-vitro management of Potato Leafroll Virus (PLRV) by using active material of different natural products

Tahsin Shoala Shoala, Ahmed Kamal El-Attar, Ahmed Ibrahim Abd El Maksoud.

Abstract
Background and aim: Potato Leafroll Virus (PLRV) is considered as one of the most important potato pathogenic virus worldwide. PLRV belongs to genus Potyvirus, transmitted by aphids and widespread in most potato growing areas globally, that causes huge losses in potato crop production. PLRV management became a challenge due to the economic importance of potato (Solanum tuberosum L.), the fourth largest staple crop in the world. Our research was aiming to manage PLRV In-Vitro. Aim: Our research was aiming to manage PLRV In-Vitro. Results: Infected potato plants (Solanum tuberosum L. var. slany) were externally sterilized and cultured in Murashige and Skoog media (MS media). Four weeks later, potato plants were tested for PLRV using RT-PCR technique, and all of the plants were PLRV positive. MS media were prepared, autoclaved, and active natural materials (Glycyrrhizic acid ammonium salt (GAS), Glycyrrhizic Acid (GA), Curcumin (CU) and Rosmarinic Acid (RA)) were sterilized and added to MS media at different concentrations.Treated potato plants with Glycyrrhizic acid ammonium salt (2ng, 4ng, and 8 ng/ml MS media) showed that the presence of PLRV in concentration 2ng/ ml MS media and the other concentrations were negatively diagnosed for PLRV. However, Treated potato plantlets with curcumin showed that PLRV present in concentrations 2ng/ ml in the MS media but treated potato plants with concentration 4ng and8 ng/ml in the MS media were negative to PLRV. Moreover, treated potato plants with Rosmarinic acid and Glycyrrhizic acid showed that the presence of PLRV in all concentrations (2ng, 4ng, 8ng/ ml in the MS media). Conclusion: In-Vitro application of Glycyrrhizic acid ammonium salt and curcumin at higher concentrations suppressed or decreased PLRV compared to low concentrations and control.

Key words: Key words: Potato, PLRV, Natural compounds, Tissue culture, Glycyrrhizic acid, Glycyrrhizic acid ammonium salt, Rosamrinic acid, Curcumin


 
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How to Cite this Article
Pubmed Style

Shoala TS, El-Attar AK, Maksoud AIAE. In-vitro management of Potato Leafroll Virus (PLRV) by using active material of different natural products. JVS. 2020; 7(1): 19-31.


Web Style

Shoala TS, El-Attar AK, Maksoud AIAE. In-vitro management of Potato Leafroll Virus (PLRV) by using active material of different natural products. http://www.j-virol-sci.com/?mno=59115 [Access: December 22, 2020].


AMA (American Medical Association) Style

Shoala TS, El-Attar AK, Maksoud AIAE. In-vitro management of Potato Leafroll Virus (PLRV) by using active material of different natural products. JVS. 2020; 7(1): 19-31.



Vancouver/ICMJE Style

Shoala TS, El-Attar AK, Maksoud AIAE. In-vitro management of Potato Leafroll Virus (PLRV) by using active material of different natural products. JVS. (2020), [cited December 22, 2020]; 7(1): 19-31.



Harvard Style

Shoala, T. S., El-Attar, . A. K. & Maksoud, . A. I. A. E. (2020) In-vitro management of Potato Leafroll Virus (PLRV) by using active material of different natural products. JVS, 7 (1), 19-31.



Turabian Style

Shoala, Tahsin Shoala, Ahmed Kamal El-Attar, and Ahmed Ibrahim Abd El Maksoud. 2020. In-vitro management of Potato Leafroll Virus (PLRV) by using active material of different natural products. Journal of Virological Sciences, 7 (1), 19-31.



Chicago Style

Shoala, Tahsin Shoala, Ahmed Kamal El-Attar, and Ahmed Ibrahim Abd El Maksoud. "In-vitro management of Potato Leafroll Virus (PLRV) by using active material of different natural products." Journal of Virological Sciences 7 (2020), 19-31.



MLA (The Modern Language Association) Style

Shoala, Tahsin Shoala, Ahmed Kamal El-Attar, and Ahmed Ibrahim Abd El Maksoud. "In-vitro management of Potato Leafroll Virus (PLRV) by using active material of different natural products." Journal of Virological Sciences 7.1 (2020), 19-31. Print.



APA (American Psychological Association) Style

Shoala, T. S., El-Attar, . A. K. & Maksoud, . A. I. A. E. (2020) In-vitro management of Potato Leafroll Virus (PLRV) by using active material of different natural products. Journal of Virological Sciences, 7 (1), 19-31.








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