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Induction Tomato Ring Spot Virus Resistant Plants using the Chemical Mutagens (NaN3 and EMS)

huda sayed darwish, om hashem mohammed el banna, mohamed said abbas, hoda mahmoud waziri, maisa anwar awad.

Abstract
Background
Tomato (Solanum lycopersicon) is affected by many biotic and abiotic stresses which became major limiting factors for growth, fruit yield quality and quantity. Tomato Ringspot Virus (ToRSV) is considered one of the most devastating members of the genus Nepovirus and distributed in most parts of the world and can infect herbaceous, ornamental and woody species including tomato, tobacco, grape, apple, peach, cherry, apricot and raspberry. Both of Sodium Azide (NaN3) and ethyl methanesulfonate (EMS) are known as chemical mutagens.
Objective
Study the effect of chemical mutagens (NaN3and EMS) on the resistance of (ToRSV) on two different tomato cultivars and characterization induction mutagens by ISSR molecular method.
Methods
ToRSV was isolated from infected tomato, grapevine and pelargonium plants and detected using Double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA). Host range study was carried out using mechanical inoculation into fourteen different diagnostic host plant species and cultivars belonging to 6 families. Tomato seeds were treated with four different concentration of NaN3and EMS (1.0, 2.0, 3.0 and 4.0 mM) and (0.3%, 0.4%, 0.5%and 0.6%) respectively, then planted into a tissue culture media, induced plants were checked for resistance to ToRSV by ELISA. ISSR reaction was used to confirm the DNA variations.
Results
ToRSV was transmitted mechanically into11 plant species and cvs., belonging to 5 families while 6 plant species and cvs. from 4 families gave negative results with DAS-ELISA and were symptomless. Treated plant with different concentrations of NaN3and EMS showed that high concentrations of both chemicals led to death of plantlets while, concentrations 3.0 and 4.0 mM of NaN3 and EMS 0.4%, 0.5% showed no symptoms of ToRSV in spite of concentrations 1mM and 2mM of NaN3 and 0.3% of EMS were showing symptoms and gave positive results with ELISA. EMS (0.40%) treated tomato cv. CR and 3mM NaN3 treated tomato cv. SSB revealed 3 unique fragments with 98-A ISSR primer, 3 unique fragments appeared in SSB cv. treated with 3mM NaN3 with 98-B ISSR primer, and E-9 ISSR primer revealed 6 fragments in EMS (0.40%) treated CR cv. and 5 fragments in 3mM NaN3 treated SSB cv. Indicting changes in the genetic make-up of the treated tomato plants. No similar fragments were observed in the control samples.
Conclusion
NaN3 at 3mM concentration and EMS at 0.4% concentration gave the highest effect in resistance to ToRSV on tomato and revealed in different unique fragments in ISSR PCR reaction, without harmful effect on the produced plants.

Key words: Keywords: Tomato, ToRSV, DAS-ELISA, Chemical mutagenesis, NaN3, EMS, ISSR.


 
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How to Cite this Article
Pubmed Style

darwish hs, banna ohme, abbas ms, waziri hm, awad ma, . Induction Tomato Ring Spot Virus Resistant Plants using the Chemical Mutagens (NaN3 and EMS). JVS. 2020; 7(1): 1-18.


Web Style

darwish hs, banna ohme, abbas ms, waziri hm, awad ma, . Induction Tomato Ring Spot Virus Resistant Plants using the Chemical Mutagens (NaN3 and EMS). http://www.j-virol-sci.com/?mno=53118 [Access: December 22, 2020].


AMA (American Medical Association) Style

darwish hs, banna ohme, abbas ms, waziri hm, awad ma, . Induction Tomato Ring Spot Virus Resistant Plants using the Chemical Mutagens (NaN3 and EMS). JVS. 2020; 7(1): 1-18.



Vancouver/ICMJE Style

darwish hs, banna ohme, abbas ms, waziri hm, awad ma, . Induction Tomato Ring Spot Virus Resistant Plants using the Chemical Mutagens (NaN3 and EMS). JVS. (2020), [cited December 22, 2020]; 7(1): 1-18.



Harvard Style

darwish, h. s., banna, o. h. m. e., abbas, m. s., waziri, h. m., awad, m. a. & (2020) Induction Tomato Ring Spot Virus Resistant Plants using the Chemical Mutagens (NaN3 and EMS). JVS, 7 (1), 1-18.



Turabian Style

darwish, huda sayed, om hashem mohammed el banna, mohamed said abbas, hoda mahmoud waziri, maisa anwar awad, and . 2020. Induction Tomato Ring Spot Virus Resistant Plants using the Chemical Mutagens (NaN3 and EMS). Journal of Virological Sciences, 7 (1), 1-18.



Chicago Style

darwish, huda sayed, om hashem mohammed el banna, mohamed said abbas, hoda mahmoud waziri, maisa anwar awad, and . "Induction Tomato Ring Spot Virus Resistant Plants using the Chemical Mutagens (NaN3 and EMS)." Journal of Virological Sciences 7 (2020), 1-18.



MLA (The Modern Language Association) Style

darwish, huda sayed, om hashem mohammed el banna, mohamed said abbas, hoda mahmoud waziri, maisa anwar awad, and . "Induction Tomato Ring Spot Virus Resistant Plants using the Chemical Mutagens (NaN3 and EMS)." Journal of Virological Sciences 7.1 (2020), 1-18. Print.



APA (American Psychological Association) Style

darwish, h. s., banna, o. h. m. e., abbas, m. s., waziri, h. m., awad, m. a. & (2020) Induction Tomato Ring Spot Virus Resistant Plants using the Chemical Mutagens (NaN3 and EMS). Journal of Virological Sciences, 7 (1), 1-18.








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