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Original Article 
JVS. 2019; 6(1): 83-92


Formulation and evaluation of inactivated lumpy skin disease virus vaccine using ISA 50 and ISA 206 adjuvants.

youssef abdallah elgharbawy.

Abstract
Lumpy skin disease (LSD) is one of the most serious livestock diseases in Egypt since 1988 when the disease spread widely all over the country. since that attenuated sheep pox virus (SPV) used for vaccination cattle flocks against LSD due to immunological relationship between LSD and SPV. There have been numerous attempts to produce live attenuated LSD vaccine but it has stopped due to the seriousness of the disease's return to its virulence and the post vaccinal reaction of the vaccine so we prepare inactivated LSD virus(LSDV) vaccine to immunize the cattle with safe vaccine .Ismailia isolate of LSD was propagated and titrated on Madin Darby Bovine Kidney Cells (MDBK) till reach the highest titer (10 5.5 TCID50/ml). The inactivation process was performed using Bromoethylamine hydro bromide (BEI) that did not affect the immunogenic characterization of the LSDV and the process last for 18 hours with 1% concentration of BEI at 37c. The inactivated LSDV tested on MDBK cells and the animals free of antibodies for residual live virus and determination of minimal protective dose (MPD) of prepared inactivated LSDV. The MPD was 1.5 ml of the inactivated virus and the vaccine was prepared by adding equal volume of the adjuvants (Montanide ISA 50 and Montanide ISA 206 ) to the total quantity 3ml of the vaccine. The prepared vaccine tested for determination of immunological response against the vaccine with ISA 50 and ISA 206 adjuvants and the results was compared. The results of cellular and humoral immune response and the challenge test showed that using inactivated LSDV vaccine improved by Montanide ISA 206 elevate the protective immunity level and considered more safe and avoid viral reversion and transmission of the virus by the blood feeding arthropods.

Key words: Lumpy skin disease adjuvants inactivated vaccine


 
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How to Cite this Article
Pubmed Style

elgharbawy ya, . Formulation and evaluation of inactivated lumpy skin disease virus vaccine using ISA 50 and ISA 206 adjuvants.. JVS. 2019; 6(1): 83-92.


Web Style

elgharbawy ya, . Formulation and evaluation of inactivated lumpy skin disease virus vaccine using ISA 50 and ISA 206 adjuvants.. http://www.j-virol-sci.com/?mno=6722 [Access: January 19, 2020].


AMA (American Medical Association) Style

elgharbawy ya, . Formulation and evaluation of inactivated lumpy skin disease virus vaccine using ISA 50 and ISA 206 adjuvants.. JVS. 2019; 6(1): 83-92.



Vancouver/ICMJE Style

elgharbawy ya, . Formulation and evaluation of inactivated lumpy skin disease virus vaccine using ISA 50 and ISA 206 adjuvants.. JVS. (2019), [cited January 19, 2020]; 6(1): 83-92.



Harvard Style

elgharbawy, y. a. & (2019) Formulation and evaluation of inactivated lumpy skin disease virus vaccine using ISA 50 and ISA 206 adjuvants.. JVS, 6 (1), 83-92.



Turabian Style

elgharbawy, youssef abdallah, and . 2019. Formulation and evaluation of inactivated lumpy skin disease virus vaccine using ISA 50 and ISA 206 adjuvants.. Journal of Virological Sciences, 6 (1), 83-92.



Chicago Style

elgharbawy, youssef abdallah, and . "Formulation and evaluation of inactivated lumpy skin disease virus vaccine using ISA 50 and ISA 206 adjuvants.." Journal of Virological Sciences 6 (2019), 83-92.



MLA (The Modern Language Association) Style

elgharbawy, youssef abdallah, and . "Formulation and evaluation of inactivated lumpy skin disease virus vaccine using ISA 50 and ISA 206 adjuvants.." Journal of Virological Sciences 6.1 (2019), 83-92. Print.



APA (American Psychological Association) Style

elgharbawy, y. a. & (2019) Formulation and evaluation of inactivated lumpy skin disease virus vaccine using ISA 50 and ISA 206 adjuvants.. Journal of Virological Sciences, 6 (1), 83-92.








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