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Original Research (Original Article) 
JVS. 2019; 5(1): 86-96
doi: 30658


Molecular Detection of Human Papilloma Virus Genotypes in Children with Focal Epithelial Hyperplasia in Khartoum State.

Samira Munir Bolis, Magda Mahmmoud, Salah El din Gumma, Naser Eldin Bilal, Isam El Khidir.

Abstract
Background: Focal epithelial hyperplasia (FEH) is a common oral infection which is usually missed in the diagnosis because it is symptomless and its lesions can regress by time. On the other hand there is no cell culture for Human papilloma viruses (HPVs).
Aim: Detection of HPV genotypes assocciated with FEH in childern aged three to fourteen years old.
Methods: Fourty seven biopsies and oral smears were collected from Children (3 -14) years old, who attended the pediatric dental clinics of Faculty of Dentistry, University of Khartoum and Khartoum Dental Teaching Hospital, clinically diagnosed by the dental clincian as FEH cases and their guardian agreed to participate. The study used the multiplex polymerase chain reaction (PCR) to detect the most common HPV genotypes associated with FEH in children, beside the Papanicolaou (Pap.) stain to show the cytological features which are usually seen in FEH. Results were analyzed using Statistical Package for Social Science (SPSS) version 16. The Chi-Square test tested the significance difference between the HPV genotypes PCR findings and the Pap. smears. Figures were constructed using Microsoft Exel 2007.
Results: Eleven specimens 23.4% (11/47) were positive for the low risk genotypes. Twenty specimens 42.6% (20/47) were positive for genotypes 13 and 32. None of genotypes 18, 39, 56 and 59 was detected. Twenty seven specimens 57.4% (27/47) were positive for the high risk genotypes. Cytologically twenty nine samples 80.5% (29/36) which were positive for the PCR showed koilocytosis and mitosoid cells. Some specimens were positive for more than one genotype. FEH was more common in females than males. There was a signifiicant difference between the PCR/Pap. findings.
Conclusion: The most frequent genotypes which were detected were HPV 32 followed by HPV 16. HPV genotypes 31, 33, 35, 52, 58 and 66 were detected in this study although they were not mentioned in the studies conducted in FEH. Pap. Stain's and PCR sensitivity was 80.5% while the specificity was 40%.
Keywords: Human papilloma viruses, Focal epithelial hyperplasia, Children, polymerase chain reaction, Papanicolaou Stain, Khartoum State.

Key words: Keywords: Human papilloma viruses, Focal epithelial hyperplasia, Children, polymerase chain reaction, Papanicolaou Stain, Khartoum State.


 
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How to Cite this Article
Pubmed Style

Bolis SM, MM, SEdG, NEB, IEK, . Molecular Detection of Human Papilloma Virus Genotypes in Children with Focal Epithelial Hyperplasia in Khartoum State.. JVS. 2019; 5(1): 86-96. doi:30658


Web Style

Bolis SM, MM, SEdG, NEB, IEK, . Molecular Detection of Human Papilloma Virus Genotypes in Children with Focal Epithelial Hyperplasia in Khartoum State.. http://www.j-virol-sci.com/?mno=30658 [Access: March 29, 2020]. doi:30658


AMA (American Medical Association) Style

Bolis SM, MM, SEdG, NEB, IEK, . Molecular Detection of Human Papilloma Virus Genotypes in Children with Focal Epithelial Hyperplasia in Khartoum State.. JVS. 2019; 5(1): 86-96. doi:30658



Vancouver/ICMJE Style

Bolis SM, MM, SEdG, NEB, IEK, . Molecular Detection of Human Papilloma Virus Genotypes in Children with Focal Epithelial Hyperplasia in Khartoum State.. JVS. (2019), [cited March 29, 2020]; 5(1): 86-96. doi:30658



Harvard Style

Bolis, S. M., , M. M., , S. E. d. G., , N. E. B., , I. E. K. & (2019) Molecular Detection of Human Papilloma Virus Genotypes in Children with Focal Epithelial Hyperplasia in Khartoum State.. JVS, 5 (1), 86-96. doi:30658



Turabian Style

Bolis, Samira Munir, Magda Mahmmoud, Salah El din Gumma, Naser Eldin Bilal, Isam El Khidir, and . 2019. Molecular Detection of Human Papilloma Virus Genotypes in Children with Focal Epithelial Hyperplasia in Khartoum State.. Journal of Virological Sciences, 5 (1), 86-96. doi:30658



Chicago Style

Bolis, Samira Munir, Magda Mahmmoud, Salah El din Gumma, Naser Eldin Bilal, Isam El Khidir, and . "Molecular Detection of Human Papilloma Virus Genotypes in Children with Focal Epithelial Hyperplasia in Khartoum State.." Journal of Virological Sciences 5 (2019), 86-96. doi:30658



MLA (The Modern Language Association) Style

Bolis, Samira Munir, Magda Mahmmoud, Salah El din Gumma, Naser Eldin Bilal, Isam El Khidir, and . "Molecular Detection of Human Papilloma Virus Genotypes in Children with Focal Epithelial Hyperplasia in Khartoum State.." Journal of Virological Sciences 5.1 (2019), 86-96. Print. doi:30658



APA (American Psychological Association) Style

Bolis, S. M., , M. M., , S. E. d. G., , N. E. B., , I. E. K. & (2019) Molecular Detection of Human Papilloma Virus Genotypes in Children with Focal Epithelial Hyperplasia in Khartoum State.. Journal of Virological Sciences, 5 (1), 86-96. doi:30658








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