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Original Article 
JVS. 2018; 4(1): 15-23


Interfering of maternal derived antibodies with the protection of loca1l inactivated reassortant H5N1 Avian influenza vaccines with antigenic content of 300HA units in commercial broiler chickens

Maha Magdy Khedr, Reem Ahmed Suliman, Marwa Fathy Mohamed, Mounir Diab El Safty, Hussein Aly Hussein.

Abstract
ABSTACT
Background: Influenza virus is an orthomyxovirus that known as one the causes of signi´Čücant numbers of natural infections and disease, mainly infecting the upper respiratory tract, in humans, domestic pigs, horses, and numerous bird species. Maternal derived antibodies interfered the efficacy of the vaccination against avian influenza in early age in chicks.

Material and method: Eight groups of one day old commercial broiler chicks were kept in isolators along all the study. Groups 1, 2 and 3 were vaccinated with the prepared vaccine ; group 4, 5 and 6 were vaccinated with one of the imported H5N1 reassortant containing 300 HAU at 1, 5 and 10 days of age; respectively. Groups 7 and 8 were positive and negative groups for the challenge trial. Blood samples were collected weekly for 4 weeks of age and tested by HI test. Post challenge, tracheal and cloacal swabs were collected at 3, 5, 7, and 10 days and tested by both real time RT-PCR (rRT-PCR) and virus titration in SPF eggs.

Results: HI test revealed no significant difference between groups in the first 3 weeks post vaccination and group 2 showed lower significant statistical difference. Results of the challenge trial revealed 0, 14, 80 % and 0, 14 and 86 % of protection in groups 1,2,3 and groups 4, 5, and 6; respectively. RT-PCR and virus isolation revealed that all chicken groups vaccinated at 1 and 5 days of age demonstrated 100% shedding at 3, 5, 7 and 10 days post challenge. However, groups 3 and 6 which were vaccinated at 10 days of age revealed difference in shedding pattern where group 3(vaccinated with local prepared vaccine) showed 100 shedding by rRT-PCR and 100%, 60% and 60 % of the chickens in tracheal swabs and 100%, 80% and 60%in cloacal swabs when tested by virus isolation in eggs at 3, 5 and 7 days post challenge; respectively. Swabs of 10 days post challenge of group 3 were positive by rRT-PCR and negative by virus isolation. On the other hand, group 6 (vaccinated with imported vaccine) demonstrated shedding % at 3 and 5 days post challenge by both rt-RT-PCR and virus isolation were positive in 60% and 20% for tracheal and 80% and 20% for cloacal swabs ; respectively. At 7 and 10 days shedding of all chickens in group 6 were negative by both rRT-PCR and virus isolation.

Conclusion: Indeed, there is evidence of interfering of maternal antibodies to vaccination at 1, and 5 days. Also, the 300HAU of antigen in the prepared avian influenza H5N1 vaccine are not enough in reduction of virus shedding post challenge

Key words: highly pathogenic avian influenza; immunity; antigen content; vaccination; challenge.


 
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How to Cite this Article
Pubmed Style

Khedr MM, Suliman RA, Mohamed MF, Safty MDE, Hussein HA. Interfering of maternal derived antibodies with the protection of loca1l inactivated reassortant H5N1 Avian influenza vaccines with antigenic content of 300HA units in commercial broiler chickens. JVS. 2018; 4(1): 15-23.


Web Style

Khedr MM, Suliman RA, Mohamed MF, Safty MDE, Hussein HA. Interfering of maternal derived antibodies with the protection of loca1l inactivated reassortant H5N1 Avian influenza vaccines with antigenic content of 300HA units in commercial broiler chickens. http://www.j-virol-sci.com/?mno=298316 [Access: March 19, 2019].


AMA (American Medical Association) Style

Khedr MM, Suliman RA, Mohamed MF, Safty MDE, Hussein HA. Interfering of maternal derived antibodies with the protection of loca1l inactivated reassortant H5N1 Avian influenza vaccines with antigenic content of 300HA units in commercial broiler chickens. JVS. 2018; 4(1): 15-23.



Vancouver/ICMJE Style

Khedr MM, Suliman RA, Mohamed MF, Safty MDE, Hussein HA. Interfering of maternal derived antibodies with the protection of loca1l inactivated reassortant H5N1 Avian influenza vaccines with antigenic content of 300HA units in commercial broiler chickens. JVS. (2018), [cited March 19, 2019]; 4(1): 15-23.



Harvard Style

Khedr, M. M., Suliman, . R. A., Mohamed, . M. F., Safty, . M. D. E. & Hussein, . H. A. (2018) Interfering of maternal derived antibodies with the protection of loca1l inactivated reassortant H5N1 Avian influenza vaccines with antigenic content of 300HA units in commercial broiler chickens. JVS, 4 (1), 15-23.



Turabian Style

Khedr, Maha Magdy, Reem Ahmed Suliman, Marwa Fathy Mohamed, Mounir Diab El Safty, and Hussein Aly Hussein. 2018. Interfering of maternal derived antibodies with the protection of loca1l inactivated reassortant H5N1 Avian influenza vaccines with antigenic content of 300HA units in commercial broiler chickens. Journal of Virological Sciences, 4 (1), 15-23.



Chicago Style

Khedr, Maha Magdy, Reem Ahmed Suliman, Marwa Fathy Mohamed, Mounir Diab El Safty, and Hussein Aly Hussein. "Interfering of maternal derived antibodies with the protection of loca1l inactivated reassortant H5N1 Avian influenza vaccines with antigenic content of 300HA units in commercial broiler chickens." Journal of Virological Sciences 4 (2018), 15-23.



MLA (The Modern Language Association) Style

Khedr, Maha Magdy, Reem Ahmed Suliman, Marwa Fathy Mohamed, Mounir Diab El Safty, and Hussein Aly Hussein. "Interfering of maternal derived antibodies with the protection of loca1l inactivated reassortant H5N1 Avian influenza vaccines with antigenic content of 300HA units in commercial broiler chickens." Journal of Virological Sciences 4.1 (2018), 15-23. Print.



APA (American Psychological Association) Style

Khedr, M. M., Suliman, . R. A., Mohamed, . M. F., Safty, . M. D. E. & Hussein, . H. A. (2018) Interfering of maternal derived antibodies with the protection of loca1l inactivated reassortant H5N1 Avian influenza vaccines with antigenic content of 300HA units in commercial broiler chickens. Journal of Virological Sciences, 4 (1), 15-23.



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