Molecular detection and phylogenetic analysis of sheep pox virus in El Menofiya Governorate.

Ashraf Shabaan Khameis, lamya Fathi Atteya, Ayman Hmid Mansour, Heba Ahmad Abdelhady, Ashraf Ahmad Saad

Abstract
SUMMARY
Background: Sheep pox virus is a member of the Capripoxvirus genus of the Poxviridae family, which affect sheep and causes significant economic losses in El Menofiya Governorate.
Objectives: The present study was applied for isolation and molecular identification of Sheep pox virus from clinically affected sheep in El Menofiya governorate, Egypt during 2016. Additionally, the isolated agent was identiﬁed using electron microscopy (EM).
Methods: Thirty five skin lesions of nodular and crusted scab samples were collected from clinically infected SPV sheep and were prepared and isolated on chorioallantoic membrane of 9-11 days old emberyonted chicken egg for three passages. The isolated samples were identified by EM and PCR.
Results: Five samples were positive and showed a typical pock lesion., the isolated strain was identified as a sheep pox virus by EM and PCR. The virus was confirmed through sequence and phylogenetic analysis of the RPO30 gene and open reading frame (ORF) 103 genes. The sequences designated Sheep pox virus was submitted to GenBank under accession number of MF443334.
Conclusion: The molecular techniques based on the RPO30 and ORF 103 genes are efficient for the characterization and differentiation of Capripox viruses. This finding may provide new information on the epidemiology of SPV in El Menofiya governorate.

Key words: SUMMARY Background: Sheep pox virus is a member of the Capripoxvirus genus of the Poxviridae family, which affect sheep and causes significant economic losses in El Menofiya Governorate. Objectives: The present study was applied for isolation and molecular identification of Sheep pox virus from clinically affected sheep in El Menofiya governorate, Egypt during 2016. Additionally, the isolated agent was identiﬁed using electron microscopy (EM)

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