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Original Article 
JVS. 2018; 3(1): 58-67
doi: 275178


Effect of methanolic-extracted green tea on hepatitis A virus and its usage in clearing the virus from drinking Water

Dina Nadeem Abd-Elshafy.

Abstract
Background: Hepatitis A virus (HAV) causes tens of millions of human infection annually worldwide. It usually spreads by the fecal-oral route and is transmitted from person-to-person through contaminated food or water. A number of travelers arriving from Egypt were found infected with HAV upon return to their home countries, for this Egypt Travel made warned tourists traveling to Egypt to avoid eating under cooked or raw food (especially meat and eggs) or raw vegetables and drinking tap water.
Aim of work: To study potential antiviral effect of methanolic extract prepared from green tea on HAV and explore supplementing drinking water in endemic areas for the virus with such an extract to control infection.
Methods: Green tea powder was used for methanolic extraction. Cytotoxicity assay was carried out according to Aquino (1989) to identify non-cyotoxic concentrations of the prepared extract. Plaque reduction assay was performed to investigate the anti-HAV activity of the green tea extract according to Tebas (1995), evaluate its effect, to test effect of applying green tea extract against contaminated drinking water with HAV and study stability of inhibitory effect of extract on HAV in water.
Results: Green tea extract has a strong anti-HAV effect with direct effect on the viral particles. Supplementing tap water with the extract with continuous shaking every 15 minutes for 1 hour caused more reduction in the percentage of HAV plaque counts compared to allowing the supplemented water to rest for without shaking and the same effect was shown when water was freezed and tested after one and two weeks indicating stability and irreversibility of the inhibitory effect of extract on HAV in water.
Conclusion: Methanolic extract of green tea has potential effect on HAV. Based on the obtained results we strongly recommend supplementing water with small concentration of green tea extract that does not cause observed change in its color and taste for 1 hour with persistent shaking before usage in endemic areas for HAV infections.

Key words: Hepatitis A virus, Green tea, plaque reduction assay, drinking water treatment.


 
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Pubmed Style

Dina Nadeem Abd-Elshafy. Effect of methanolic-extracted green tea on hepatitis A virus and its usage in clearing the virus from drinking Water. JVS. 2018; 3(1): 58-67. doi:275178


Web Style

Dina Nadeem Abd-Elshafy. Effect of methanolic-extracted green tea on hepatitis A virus and its usage in clearing the virus from drinking Water. http://www.j-virol-sci.com/?mno=275178 [Access: December 14, 2018]. doi:275178


AMA (American Medical Association) Style

Dina Nadeem Abd-Elshafy. Effect of methanolic-extracted green tea on hepatitis A virus and its usage in clearing the virus from drinking Water. JVS. 2018; 3(1): 58-67. doi:275178



Vancouver/ICMJE Style

Dina Nadeem Abd-Elshafy. Effect of methanolic-extracted green tea on hepatitis A virus and its usage in clearing the virus from drinking Water. JVS. (2018), [cited December 14, 2018]; 3(1): 58-67. doi:275178



Harvard Style

Dina Nadeem Abd-Elshafy (2018) Effect of methanolic-extracted green tea on hepatitis A virus and its usage in clearing the virus from drinking Water. JVS, 3 (1), 58-67. doi:275178



Turabian Style

Dina Nadeem Abd-Elshafy. 2018. Effect of methanolic-extracted green tea on hepatitis A virus and its usage in clearing the virus from drinking Water. Journal of Virological Sciences, 3 (1), 58-67. doi:275178



Chicago Style

Dina Nadeem Abd-Elshafy. "Effect of methanolic-extracted green tea on hepatitis A virus and its usage in clearing the virus from drinking Water." Journal of Virological Sciences 3 (2018), 58-67. doi:275178



MLA (The Modern Language Association) Style

Dina Nadeem Abd-Elshafy. "Effect of methanolic-extracted green tea on hepatitis A virus and its usage in clearing the virus from drinking Water." Journal of Virological Sciences 3.1 (2018), 58-67. Print. doi:275178



APA (American Psychological Association) Style

Dina Nadeem Abd-Elshafy (2018) Effect of methanolic-extracted green tea on hepatitis A virus and its usage in clearing the virus from drinking Water. Journal of Virological Sciences, 3 (1), 58-67. doi:275178



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