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Online First Current Issue Archive Most Downloaded Most Accessed « Previous Article Next Article » Original Article   Open Access JVS. 2017; 2(1): 17-31 Characterization of a novel asymptomatic isolate of Tomato spotted wilt virus, Infects chrysanthemum plants in Egypt Ahmed Kamal El-Attar, Manal Ali EL-Shazly, Samah Abdel Salam Mokbel. Abstract Background: A latent infection by Tomato spotted wilt virus (TSWV) in traded chrysanthemum plants at the local level constitutes a severe threat to the Egyptian farming. Methods: The TSWV isolate in asymptomatic infected chrysanthemum plants was detected using double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA) and reverse transcription-polymerase chain reaction (RT-PCR). Host range studies were carried out by mechanical inoculation of biologically purified isolate on a set of different vegetable, ornamental and herbaceous plants. RT-PCR was used to amplify the full length nucleocapsid protein (N) gene of TSWV; the amplified 777 bp fragment was molecularly cloned and sequenced. The phylogenetic tree was constructed using the DNAMAN software. Results: The obtained results demonstrated that, this isolate of TSWV causes asymptomatic infection for chrysanthemum plants in different locations in Giza governorate. The ELISA was less efficient than the RT-PCR for the diagnoses of the latent infection. RT-PCR accurately identified 26% more positive samples without symptoms than did the DAS-ELISA test. Moreover, RT-PCR was used to confirm TSWV infection related to the development of local lesions and systemic symptoms from mechanical inoculations. The nucleotide sequence of the TSWV-N gene has been deposited in the GenBank with accession number KY569403. Phylogenetic analysis showed that the fragment sequence shared variable levels of similarity and 93.82% max identity with that of N-gene sequences from Japan (AB038341), Pakistan (KX121046), South Korea (AB910533) and Turkey (KM407603). Conclusion: The relatively low levels of sequence identity (91.7% to 93.8%) to N-gene demonstrated the release of a new isolate of TSWV in Egypt. Moreover, the study showed the susceptibility of onion and sweet potato to TSWV-isolate suggesting the chance of spread of viral infection on cultivated crops in the future. The information generated in this study will be useful for alleviating problems associated with non-specific symptoms and can be used to aid efficient management strategies directed against TSWV. Key words: Chrysanthemums; Latent infection; Tospovirus; Tomato spotted wilt virus; Nucleocapsid protein; N-gene; DAS-ELISA; RT-PCR; Cloning.   ARTICLE TOOLS Abstract PDF Fulltext Print this Article How to cite this article Export to Export to Related Records   Articles by Ahmed Kamal El-Attar Articles by Manal Ali EL-Shazly Articles by Samah Abdel Salam Mokbel on Google on Google Scholar Article Statistics   Viewed: 53 Downloaded: 30 Cited: 0 How to Cite this Article Pubmed Style El-Attar AK, EL-Shazly MA, Mokbel SAS. Characterization of a novel asymptomatic isolate of Tomato spotted wilt virus, Infects chrysanthemum plants in Egypt. JVS. 2017; 2(1): 17-31. Web Style El-Attar AK, EL-Shazly MA, Mokbel SAS. Characterization of a novel asymptomatic isolate of Tomato spotted wilt virus, Infects chrysanthemum plants in Egypt. www.scopemed.org/?mno=269890 [Access: October 17, 2017]. AMA (American Medical Association) Style El-Attar AK, EL-Shazly MA, Mokbel SAS. Characterization of a novel asymptomatic isolate of Tomato spotted wilt virus, Infects chrysanthemum plants in Egypt. JVS. 2017; 2(1): 17-31. Vancouver/ICMJE Style El-Attar AK, EL-Shazly MA, Mokbel SAS. Characterization of a novel asymptomatic isolate of Tomato spotted wilt virus, Infects chrysanthemum plants in Egypt. JVS. (2017), [cited October 17, 2017]; 2(1): 17-31. Harvard Style El-Attar, A. K., EL-Shazly, M. A. & Mokbel, S. A. S. (2017) Characterization of a novel asymptomatic isolate of Tomato spotted wilt virus, Infects chrysanthemum plants in Egypt. JVS, 2 (1), 17-31. Turabian Style El-Attar, Ahmed Kamal, Manal Ali EL-Shazly, and Samah Abdel Salam Mokbel. 2017. Characterization of a novel asymptomatic isolate of Tomato spotted wilt virus, Infects chrysanthemum plants in Egypt. Journal of Virological Sciences, 2 (1), 17-31. Chicago Style El-Attar, Ahmed Kamal, Manal Ali EL-Shazly, and Samah Abdel Salam Mokbel. "Characterization of a novel asymptomatic isolate of Tomato spotted wilt virus, Infects chrysanthemum plants in Egypt." Journal of Virological Sciences 2 (2017), 17-31. MLA (The Modern Language Association) Style El-Attar, Ahmed Kamal, Manal Ali EL-Shazly, and Samah Abdel Salam Mokbel. "Characterization of a novel asymptomatic isolate of Tomato spotted wilt virus, Infects chrysanthemum plants in Egypt." Journal of Virological Sciences 2.1 (2017), 17-31. Print. APA (American Psychological Association) Style El-Attar, A. K., EL-Shazly, M. A. & Mokbel, S. A. S. (2017) Characterization of a novel asymptomatic isolate of Tomato spotted wilt virus, Infects chrysanthemum plants in Egypt. Journal of Virological Sciences, 2 (1), 17-31.

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