ISSN 2636-2937
 

Original Research (Original Article) 
JVS. 2019; 5(1): 76-85
doi: 26749


Detection of Potato Virus M in commercial local potato seed tubers in Egypt

Allam Arafat Megahed, Badawi Abdelsalam Othman , Samar S. El Masry, Abeer A. Faiesal, Mohamed Atef Nasr Eldin .

Abstract
Background: Potato virus M (PVM) is found in potato cultivars worldwide including Egypt. Potato tubers are a common source of the virus during host cultivations. The yield loss can range from 15% to 45% and heavy losses can occur upon mixed infection with PVM and PVS or PVX.
Methods: The PVM was detected in symptomatic infected potato plants during growing seasons for the cultivated commercial local tuber seeds using Double Antibody Sandwich-Enzyme Linked Immuno-Sorbent Assay (DAS-ELISA). The PVM was mechanically isolated on potato cv. Diamant. Host range was carried out by mechanical inoculation of PVM on a set of different plant species. Electron Microscopy was used to examine the virus morphology. The cytopathic effect of the virus on tissue ultrathin section was examined by transmission electron microscope (TEM). Finally, the PVM isolate was detected using reverse transcription-polymerase chain reaction (RT-PCR) in the sprouts after dormancy breaking for the harvested potato tubers.
Results: The obtained results demonstrated that, PVM isolate causes severe symptoms on potato plants under the greenhouse conditions. The infected potato cv. Diamant gave positive DAS-ELISA result using polyclonal antibodies against PVM. Moreover, the RT-PCR confirmed PVM in tuber sprouts using coat protein gene specific primers with an expected amplicon of 520 bp. Electron Microscopy examination revealed that, the viral particles were slightly flexuous rod shape with about 680 x 13 nm in size. Ultrastructure analysis showed different degrees of chloroplast degradation in PVM-infected potato leaf tissues.
Conclusion: Occurrence of PVM was detected and confirmed in commercial potato seed tubers in Egypt using the RT-PCR technique.

Key words: Potato Virus M; potato seeds; DAS-ELISA; RT-PCR; Virus morphology; Cytopathology


 
ARTICLE TOOLS
Abstract
PDF Fulltext
Print this article Print this Article
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Allam Arafat Megahed
Articles by Badawi Abdelsalam Othman
Articles by Samar S. El Masry
Articles by Abeer A. Faiesal
Articles by Mohamed Atef Nasr Eldin
on Google
on Google Scholar
Article Statistics
 Viewed: 678
Downloaded: 0
Cited: 0


How to Cite this Article
Pubmed Style

Megahed AA, BAO, Masry SSE, Faiesal AA, , . Detection of Potato Virus M in commercial local potato seed tubers in Egypt. JVS. 2019; 5(1): 76-85. doi:26749


Web Style

Megahed AA, BAO, Masry SSE, Faiesal AA, , . Detection of Potato Virus M in commercial local potato seed tubers in Egypt. http://www.j-virol-sci.com/?mno=26749 [Access: August 19, 2019]. doi:26749


AMA (American Medical Association) Style

Megahed AA, BAO, Masry SSE, Faiesal AA, , . Detection of Potato Virus M in commercial local potato seed tubers in Egypt. JVS. 2019; 5(1): 76-85. doi:26749



Vancouver/ICMJE Style

Megahed AA, BAO, Masry SSE, Faiesal AA, , . Detection of Potato Virus M in commercial local potato seed tubers in Egypt. JVS. (2019), [cited August 19, 2019]; 5(1): 76-85. doi:26749



Harvard Style

Megahed, A. A., , B. A. O., Masry, S. S. E., Faiesal, A. A., & (2019) Detection of Potato Virus M in commercial local potato seed tubers in Egypt. JVS, 5 (1), 76-85. doi:26749



Turabian Style

Megahed, Allam Arafat, Badawi Abdelsalam Othman, Samar S. El Masry, Abeer A. Faiesal, Mohamed Atef Nasr Eldin, and . 2019. Detection of Potato Virus M in commercial local potato seed tubers in Egypt. Journal of Virological Sciences, 5 (1), 76-85. doi:26749



Chicago Style

Megahed, Allam Arafat, Badawi Abdelsalam Othman, Samar S. El Masry, Abeer A. Faiesal, Mohamed Atef Nasr Eldin, and . "Detection of Potato Virus M in commercial local potato seed tubers in Egypt." Journal of Virological Sciences 5 (2019), 76-85. doi:26749



MLA (The Modern Language Association) Style

Megahed, Allam Arafat, Badawi Abdelsalam Othman, Samar S. El Masry, Abeer A. Faiesal, Mohamed Atef Nasr Eldin, and . "Detection of Potato Virus M in commercial local potato seed tubers in Egypt." Journal of Virological Sciences 5.1 (2019), 76-85. Print. doi:26749



APA (American Psychological Association) Style

Megahed, A. A., , B. A. O., Masry, S. S. E., Faiesal, A. A., & (2019) Detection of Potato Virus M in commercial local potato seed tubers in Egypt. Journal of Virological Sciences, 5 (1), 76-85. doi:26749





Most Viewed Articles
  • Co-circulation of three different serotypes of FMD virus in Egypt
    Mahmoud talaat attia, Emad foad Elgendy, Momtaz Abdelhady Shahein, Samy Gomaa Kasem, madiha salah ibrahim
    JVS. 2017; 2(1): 102-113
    » Abstract

  • Isolation of H5 Highly Pathogenic Avian Influenza Virus from Cattle Egret (Bubulcus ibis) near Affected Broiler Chicken Flocks in Egypt
    Moustafa M. El-Shazly, Basem M. Ahmed, Ahmed A. El-Sanousi, Youssef I. Youssef
    JVS. 2017; 2(1): 62-70
    » Abstract

  • Identification and Characterization of Soybean vein necrosis virus (SVNV): A newly Isolated Thrips-Borne Tospovirus in Egypt
    Abeer Salah Abd El-Wahab, Manal Ali El-Shazly
    JVS. 2017; 1(Vol. 1, Jan. 2017): 76-90
    » Abstract

  • Elimination of Alfalfa mosaic virus (AMV) from infected potato leaves (Solanum tuberosum) cv. Ditta by embryonic calli
    Maha Ahmed Maher El-Abhar, Moustafa Ahmed Salama El-Kady, Khaled Mohamed Ghanem, Hussieny Abd-Elhak Bosila
    JVS. 2017; 1(Vol. 1, Jan. 2017): 100-113
    » Abstract

  • Prevalence of equine herpes viruses 1 and 4 in Arabian horses population in Egypt
    Soha Ibrahim Mohamed, Mohamed Abd-Elhameed Shalaby, Ayman Hany El Deeb, Sayed Ahmed Salem
    JVS. 2017; 2(1): 1-9
    » Abstract

  • Detection of equine herpesvirus type1and type 4 associated with abortion and respiratory manifestation in horses during 2016 in Cairo, Egypt
    Shimaa Mousleh Ghoniem, Ayman Hany El-Deeb, Mohamed Galal Aggour, Hussein Aly Hussein
    JVS. 2017; 2(1): 56-61
    » Abstract

  • Herpes Simplex virus type 1 persistence in water from different sources
    Dina Nadeem Abd-Elshafy, Mahmoud Mohamed Bahgat
    JVS. 2017; 2(1): 114-123
    » Abstract

  • Field application of trivalent foot and mouth disease vaccine adjuvanted with Zeolite.
    Hiam Fakhry Alhawary, Assem Mohamed abobaker, Sonia Ahmed Rizk, Akram Zakria Hegazi, Abobaker Ahmed Agoor
    JVS. 2017; 2(1): 32-41
    » Abstract

  • first isolation and identification of EHV-4 causing abortion in Egypt
    Ahmed Fawzy Afify, Basem M. Ahmed, Sayed Ahmed Salem, Ahmed Abd El-Ghani El-Sanousi, Mohamed Abd El-Hameed Shalaby
    JVS. 2017; 2(1): 92-101
    » Abstract

  • ISOLATION AND IDENTIFICATION OF LUMPY SKIN DISEASE VIRUS IN CATTLE IN KALUBEYA GOVERNORATE
    Lamya Ateya Fathy Ateya, Ahmed Said Ahmed, Ayman Hamed Mansour, Ashraf Khames, Heba Ahmed Abdel Hady
    JVS. 2017; 1(Vol. 1, Jan. 2017): 12-19
    » Abstract

  • Most Downloaded
  • Standardization of Quality Control Protocol for Evaluation of Recombinant HVT-H5 Vaccine
    ola youssef mohamed, Ayman Hany Mahmoud, Samir Abdel- Moez Nasif, Ahmed Abdel El-Ghani El-Sanousi
    JVS. 2017; 1(Vol. 1, Jan. 2017): 27-39
    » Abstract

  • ISOLATION AND IDENTIFICATION OF LUMPY SKIN DISEASE VIRUS IN CATTLE IN KALUBEYA GOVERNORATE
    Lamya Ateya Fathy Ateya, Ahmed Said Ahmed, Ayman Hamed Mansour, Ashraf Khames, Heba Ahmed Abdel Hady
    JVS. 2017; 1(Vol. 1, Jan. 2017): 12-19
    » Abstract

  • Field application of trivalent foot and mouth disease vaccine adjuvanted with Zeolite.
    Hiam Fakhry Alhawary, Assem Mohamed abobaker, Sonia Ahmed Rizk, Akram Zakria Hegazi, Abobaker Ahmed Agoor
    JVS. 2017; 2(1): 32-41
    » Abstract

  • Identification and Molecular Characterization of a Phytoplasma Associated With Lemon Witches’-Broom in Egypt
    Rasha M. Mahrous, Ahmed K. El-Attar, Ahlam A. ALWatban, Sohair I. EL-Afifi, Nagwa M. Aref
    JVS. 2018; 3(1): 30-41
    » Abstract » doi: 283423

  • Detection of equine herpesvirus type1and type 4 associated with abortion and respiratory manifestation in horses during 2016 in Cairo, Egypt
    Shimaa Mousleh Ghoniem, Ayman Hany El-Deeb, Mohamed Galal Aggour, Hussein Aly Hussein
    JVS. 2017; 2(1): 56-61
    » Abstract

  • Very virulent IBDV strain Egypt/IBDV/Behera2011: Macroscopic and microscopic lesions accompanied with induced mortalities in SPF-chicks
    Reem A. Suliman, Basem M. Ahmed, Mounir M. El-Safty, Hussein Aly Hussein
    JVS. 2017; 2(1): 79-91
    » Abstract

  • Genetic and Antigenic Characterization of Infectious Bronchitis Virus Strains in Egypt
    Sara Mahmoud Ameen, Abdullah Selim, Ahmed El-Sanousi, Mohamed Shalaby
    JVS. 2017; 2(1): 42-55
    » Abstract

  • Genetic characterization of rabbit hemorrhagic disease virus from naturally-infected rabbits in Sharkia governorate, Egypt
    Naglaa F.S. Awad, Gamelat K.F. Kotb
    JVS. 2018; 3(1): 10-19
    » Abstract » doi: 282147

  • Incidence, serological and molecular characterization of Potato virus S from commercial potato in Egypt
    Salama M El-saghir
    JVS. 2017; 1(Vol. 1, Jan. 2017): 67-75
    » Abstract

  • Elimination of Alfalfa mosaic virus (AMV) from infected potato leaves (Solanum tuberosum) cv. Ditta by embryonic calli
    Maha Ahmed Maher El-Abhar, Moustafa Ahmed Salama El-Kady, Khaled Mohamed Ghanem, Hussieny Abd-Elhak Bosila
    JVS. 2017; 1(Vol. 1, Jan. 2017): 100-113
    » Abstract

  • Most Cited Articles