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Original Article Open Access
JVS. 2017; 2(1): 71-78


Detection of Epstein-Barr Virus and Herpes Simplex Virus Type-1 in Tonsils or Adenoids

Eman Abdelfatah Abushady, Bahaa mohamed Abd El-Salam, Azza ahmed mira, magda abdel-latif Mohamed.

Abstract
Background: Recurrent tonsillitis has been the subject of frequent investigations, mechanisms by which some patients develop recurring tonsillitis still unclear. Some studies showed that tonsils and adenoids may act as a reservoir for some herpes viruses including EBV and HSV-1. The aim of this study is to verify whether tonsils and adenoids may harbor EBV, HSV-1 in latent state and their reactivation may lead to recurrent tonsillitis.
Material and methods: for these reasons 50 samples (40 tonsils and 10 adenoids) and their 50 swabs were taken to detect viral shedding in throat are being used to demonstrate the presence of the EBV and HSV-1 by Multiplex polymerase chain reaction (M- PCR) and tissue culture.
Results: Regarding HSV-1; all samples gave negative result (0%) using multiplex PCR for tonsils and adenoids. Tissue culture was used to isolate HSV-1 in all samples; none of them gave the characteristic cytopathogenic effect of HSV-1. Regarding EBV M-PCR of tonsils and adenoids 9 out of 50 (18%) was positive and their swabs were negative. The prevalence of EBV infection in adenoids was higher than in tonsils, but the difference is statistically non significant. Regarding sex the prevalence of EBV infection the difference was statistically non significant and it was also so for the mean age.
Conclusions: EBV present in tonsils in latent stage and may have a role in recurrent tonsillitis and systemic diseases.
Key words: Tonsils and adenoid, latent EBV &HSV-1, by M-PCR and tissue culture.

Key words: Key words: Tonsils and adenoid, latent EBV &HSV-1, by M-PCR and tissue culture.


 
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How to Cite this Article
Pubmed Style

Abushady EA, El-Salam BmA, mira Aa, Mohamed ma. Detection of Epstein-Barr Virus and Herpes Simplex Virus Type-1 in Tonsils or Adenoids. JVS. 2017; 2(1): 71-78.


Web Style

Abushady EA, El-Salam BmA, mira Aa, Mohamed ma. Detection of Epstein-Barr Virus and Herpes Simplex Virus Type-1 in Tonsils or Adenoids. http://www.j-virol-sci.com/?mno=267173 [Access: December 18, 2017].


AMA (American Medical Association) Style

Abushady EA, El-Salam BmA, mira Aa, Mohamed ma. Detection of Epstein-Barr Virus and Herpes Simplex Virus Type-1 in Tonsils or Adenoids. JVS. 2017; 2(1): 71-78.



Vancouver/ICMJE Style

Abushady EA, El-Salam BmA, mira Aa, Mohamed ma. Detection of Epstein-Barr Virus and Herpes Simplex Virus Type-1 in Tonsils or Adenoids. JVS. (2017), [cited December 18, 2017]; 2(1): 71-78.



Harvard Style

Abushady, E. A., El-Salam, B. m. A., mira, A. a. & Mohamed, m. a. (2017) Detection of Epstein-Barr Virus and Herpes Simplex Virus Type-1 in Tonsils or Adenoids. JVS, 2 (1), 71-78.



Turabian Style

Abushady, Eman Abdelfatah, Bahaa mohamed Abd El-Salam, Azza ahmed mira, and magda abdel-latif Mohamed. 2017. Detection of Epstein-Barr Virus and Herpes Simplex Virus Type-1 in Tonsils or Adenoids. Journal of Virological Sciences, 2 (1), 71-78.



Chicago Style

Abushady, Eman Abdelfatah, Bahaa mohamed Abd El-Salam, Azza ahmed mira, and magda abdel-latif Mohamed. "Detection of Epstein-Barr Virus and Herpes Simplex Virus Type-1 in Tonsils or Adenoids." Journal of Virological Sciences 2 (2017), 71-78.



MLA (The Modern Language Association) Style

Abushady, Eman Abdelfatah, Bahaa mohamed Abd El-Salam, Azza ahmed mira, and magda abdel-latif Mohamed. "Detection of Epstein-Barr Virus and Herpes Simplex Virus Type-1 in Tonsils or Adenoids." Journal of Virological Sciences 2.1 (2017), 71-78. Print.



APA (American Psychological Association) Style

Abushady, E. A., El-Salam, B. m. A., mira, A. a. & Mohamed, m. a. (2017) Detection of Epstein-Barr Virus and Herpes Simplex Virus Type-1 in Tonsils or Adenoids. Journal of Virological Sciences, 2 (1), 71-78.



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