Detection of equine herpesvirus type1and type 4 associated with abortion and respiratory manifestation in horses during 2016 in Cairo, Egypt

Shimaa Mousleh Ghoniem, Ayman Hany El-Deeb, Mohamed Galal Aggour, Hussein Aly Hussein

Abstract
Background: Equine herpesvirus 1 (EHV-1) and Equine herpesvirus 4 (EHV-4) are endemic in equine population worldwide. EHV-1 is a major cause of abortion in pregnant mares, also it can cause neurological disorders in horses and respiratory disease in young horses. EHV- 4 is an important equine virus which cause respiratory disease and occasionally abortion.
Methods: In the present study, 75 serum samples collected from aborted mares, growing foals and, adult horses suffered from respiratory manifestation were tested using indirect ELISA technique. A total of 33 nasal swabs and 4 vaginal swabs were tested using real time PCR.
Results: Out of 75 tested serum samples, 56 samples were positive for the presence of antibodies against EHVs. EHV-1 DNA was detected in two vaginal swabs, one nasal swab of adult horse and four nasal swabs of growing foals. EHV-4 DNA was detected in one vaginal swab and one nasal swab from growing foal.
Conclusion: The study reports the infection with two types of EHV and their continuous circulation among Egyptian horses during 2016.

Key words: Key words: Equine herpesviruses, Epidemiology, ELISA, Real time PCR

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