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Original Article Open Access


Prevalence of equine herpes viruses 1 and 4 in Arabian horses population in Egypt

Soha Ibrahim Mohamed, Mohamed Abd-Elhameed Shalaby, Ayman Hany El Deeb, Sayed Ahmed Salem.

Abstract
Objectives :Equid herpes viruses 1 and 4 are the most important viruses affecting horses of all ages and other members of Equidae family (donkeys and mules) causing extensive economic losses from abortion , neonatal foal death and respiratory diseases. Infection caused by EHV-1,4 typically result in establishment of latent infection within the first weeks or months of life with subsequent viral reactivation causing clinical disease and viral shedding during periods of stress.
Methods :Three hundred sera sample were examined for the presence of specific antibodies against equine herpes virus 1 and 4 in horses , mules and donkeys from 6 governorates in Egypt by indirect ELISA test. Detection of EHV-1 and EHV-4 by nested multiplex PCR and sequence analysis .Isolation of EHV-1 on tissue culture (MDBK cells) and identification by indirect florescent antibody technique (IFAT).
Results : By ELISA test , 234 samples were found positive for the presence of EHV-1 and 4 antibodies. By PCR , one sample was found positive for EHV-1 and three samples for EHV-4. Sequence analysis of the amplified nested PCR products of representative EHV strains further confirmed the virus identity. In this work, we isolated EHV-1 on tissue culture(MDBK cells)from tissue samples including (liver, lung, kidney, spleen ,heart and brain of aborted fetuses ) collected from Arabian horses located in Cairo, Egypt . Sample was propagated for three passages on MDBK cells showing the CPE after 24 72 hours the characteristic grape like clusters of cells which are rounded and aggregated together in a separate manner and gradually increased till 70- 80% of the sheet was completely detached .Identification of positive EHV-1 isolate was done by indirect florescent antibody technique (IFAT) ; the result showed apple green fluorescent granules.
Conclusion: This work was planned to detect cause of many cases of abortion among horses in Egypt .The PCR technique proved to be more sensitive , rapid and efficient for diagnosis of EHV -1 &4 infection , while ELISA technique is more sensitive for detection of EHV-1 and 4 antibodies . This study will help in planning for vaccination against Egyptian isolates .

Key words: Arabian horses, Egypt, EHV-1 , EHV-4 , IFAT, nested multiplex PCR, ELISA.


 
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How to Cite this Article
Pubmed Style

Mohamed SI, Shalaby MA, Deeb AHE, Salem SA. Prevalence of equine herpes viruses 1 and 4 in Arabian horses population in Egypt. JVS. 2017; 2(1): 1-9.


Web Style

Mohamed SI, Shalaby MA, Deeb AHE, Salem SA. Prevalence of equine herpes viruses 1 and 4 in Arabian horses population in Egypt. http://www.j-virol-sci.com/?mno=258783 [Access: December 18, 2017].


AMA (American Medical Association) Style

Mohamed SI, Shalaby MA, Deeb AHE, Salem SA. Prevalence of equine herpes viruses 1 and 4 in Arabian horses population in Egypt. JVS. 2017; 2(1): 1-9.



Vancouver/ICMJE Style

Mohamed SI, Shalaby MA, Deeb AHE, Salem SA. Prevalence of equine herpes viruses 1 and 4 in Arabian horses population in Egypt. JVS. (2017), [cited December 18, 2017]; 2(1): 1-9.



Harvard Style

Mohamed, S. I., Shalaby, M. A., Deeb, A. H. E. & Salem, S. A. (2017) Prevalence of equine herpes viruses 1 and 4 in Arabian horses population in Egypt. JVS, 2 (1), 1-9.



Turabian Style

Mohamed, Soha Ibrahim, Mohamed Abd-Elhameed Shalaby, Ayman Hany El Deeb, and Sayed Ahmed Salem. 2017. Prevalence of equine herpes viruses 1 and 4 in Arabian horses population in Egypt. Journal of Virological Sciences, 2 (1), 1-9.



Chicago Style

Mohamed, Soha Ibrahim, Mohamed Abd-Elhameed Shalaby, Ayman Hany El Deeb, and Sayed Ahmed Salem. "Prevalence of equine herpes viruses 1 and 4 in Arabian horses population in Egypt." Journal of Virological Sciences 2 (2017), 1-9.



MLA (The Modern Language Association) Style

Mohamed, Soha Ibrahim, Mohamed Abd-Elhameed Shalaby, Ayman Hany El Deeb, and Sayed Ahmed Salem. "Prevalence of equine herpes viruses 1 and 4 in Arabian horses population in Egypt." Journal of Virological Sciences 2.1 (2017), 1-9. Print.



APA (American Psychological Association) Style

Mohamed, S. I., Shalaby, M. A., Deeb, A. H. E. & Salem, S. A. (2017) Prevalence of equine herpes viruses 1 and 4 in Arabian horses population in Egypt. Journal of Virological Sciences, 2 (1), 1-9.



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