ISSN 2636-2937
 

Original Research (Original Article) 
JVS. 2019; 5(1): 134-141
doi: 2489


Molecular characterization of IBDV in Egypt,2015

Aml Saad El-Saadany, Ayman Hany El-Deeb, Hussien Aly Hussien.

Abstract
Background: Infectious bursal disease virus (IBDV) remerged frequently in Egypt, causing serious economic losses. In Egypt very virulent (vv) IBDV strains are annually reported since its first introduction in 1989. Variant IBDV strains were also reported in few studies. To date, IBDV associated with breaks has been characterized in flocks which were vaccinated with classical IBDV vaccines and demonstrated IBD characteristic lesions.
Objectives: In present study the aim was to molecular characterize IBDV field strains detected in flocks located in 4 governorates. Also, a phylogenetic analysis based on the sequence of the hyper variable region of VP2 gene was carried out.
Methods: Forty field bursa samples were collected from different localities. IBDV was detected using RT-PCR. Sequence analysis of the variable region of VP2 gene purified from the PCR product. Comparative phylogenetic analysis based on the sequence of the hyper variable region of VP2 gene as well as phylogenetic tree was constructed along with local and reference IBDV strains using DNAstar – MegAlign” software.
Results: Eight out of 40 samples were positive for IBDV with RT-PCR, Comparative phylogenetic analysis based on the sequence of the hyper variable region of VP2 gene showed that the characterized strains were closely related to IBDV Giza 2008 strains.
Conclusion: this study reports the continuous circulation of vvIBDV strains in vaccinated flocks 4 governorates “Giza, Sharkia, Behira, and Dakahlia”, in Egypt during year 2015. There are need to review the control strategy and the vaccination failure of IBDV in broiler flocks specially the extensive abuse of using classical vaccines.

Key words: IBDV, RT-PCR, VP2, Sequencing, phylogenetic analysis


 
ARTICLE TOOLS
Abstract
PDF Fulltext
Print this article Print this Article
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Aml Saad El-Saadany
Articles by Ayman Hany El-Deeb
Articles by Hussien Aly Hussien
on Google
on Google Scholar
Article Statistics
 Viewed: 1154
Downloaded: 19
Cited: 0


How to Cite this Article
Pubmed Style

El-Saadany AS, El-Deeb AH, Hussien HA, . Molecular characterization of IBDV in Egypt,2015. JVS. 2019; 5(1): 134-141. doi:2489


Web Style

El-Saadany AS, El-Deeb AH, Hussien HA, . Molecular characterization of IBDV in Egypt,2015. http://www.j-virol-sci.com/?mno=2489 [Access: June 27, 2019]. doi:2489


AMA (American Medical Association) Style

El-Saadany AS, El-Deeb AH, Hussien HA, . Molecular characterization of IBDV in Egypt,2015. JVS. 2019; 5(1): 134-141. doi:2489



Vancouver/ICMJE Style

El-Saadany AS, El-Deeb AH, Hussien HA, . Molecular characterization of IBDV in Egypt,2015. JVS. (2019), [cited June 27, 2019]; 5(1): 134-141. doi:2489



Harvard Style

El-Saadany, A. S., El-Deeb, . A. H., Hussien, . H. A. & (2019) Molecular characterization of IBDV in Egypt,2015. JVS, 5 (1), 134-141. doi:2489



Turabian Style

El-Saadany, Aml Saad, Ayman Hany El-Deeb, Hussien Aly Hussien, and . 2019. Molecular characterization of IBDV in Egypt,2015. Journal of Virological Sciences, 5 (1), 134-141. doi:2489



Chicago Style

El-Saadany, Aml Saad, Ayman Hany El-Deeb, Hussien Aly Hussien, and . "Molecular characterization of IBDV in Egypt,2015." Journal of Virological Sciences 5 (2019), 134-141. doi:2489



MLA (The Modern Language Association) Style

El-Saadany, Aml Saad, Ayman Hany El-Deeb, Hussien Aly Hussien, and . "Molecular characterization of IBDV in Egypt,2015." Journal of Virological Sciences 5.1 (2019), 134-141. Print. doi:2489



APA (American Psychological Association) Style

El-Saadany, A. S., El-Deeb, . A. H., Hussien, . H. A. & (2019) Molecular characterization of IBDV in Egypt,2015. Journal of Virological Sciences, 5 (1), 134-141. doi:2489





Most Viewed Articles
  • Co-circulation of three different serotypes of FMD virus in Egypt
    Mahmoud talaat attia, Emad foad Elgendy, Momtaz Abdelhady Shahein, Samy Gomaa Kasem, madiha salah ibrahim
    JVS. 2017; 2(1): 102-113
    » Abstract

  • Isolation of H5 Highly Pathogenic Avian Influenza Virus from Cattle Egret (Bubulcus ibis) near Affected Broiler Chicken Flocks in Egypt
    Moustafa M. El-Shazly, Basem M. Ahmed, Ahmed A. El-Sanousi, Youssef I. Youssef
    JVS. 2017; 2(1): 62-70
    » Abstract

  • Identification and Characterization of Soybean vein necrosis virus (SVNV): A newly Isolated Thrips-Borne Tospovirus in Egypt
    Abeer Salah Abd El-Wahab, Manal Ali El-Shazly
    JVS. 2017; 1(Vol. 1, Jan. 2017): 76-90
    » Abstract

  • Elimination of Alfalfa mosaic virus (AMV) from infected potato leaves (Solanum tuberosum) cv. Ditta by embryonic calli
    Maha Ahmed Maher El-Abhar, Moustafa Ahmed Salama El-Kady, Khaled Mohamed Ghanem, Hussieny Abd-Elhak Bosila
    JVS. 2017; 1(Vol. 1, Jan. 2017): 100-113
    » Abstract

  • Herpes Simplex virus type 1 persistence in water from different sources
    Dina Nadeem Abd-Elshafy, Mahmoud Mohamed Bahgat
    JVS. 2017; 2(1): 114-123
    » Abstract

  • Field application of trivalent foot and mouth disease vaccine adjuvanted with Zeolite.
    Hiam Fakhry Alhawary, Assem Mohamed abobaker, Sonia Ahmed Rizk, Akram Zakria Hegazi, Abobaker Ahmed Agoor
    JVS. 2017; 2(1): 32-41
    » Abstract

  • Prevalence of equine herpes viruses 1 and 4 in Arabian horses population in Egypt
    Soha Ibrahim Mohamed, Mohamed Abd-Elhameed Shalaby, Ayman Hany El Deeb, Sayed Ahmed Salem
    JVS. 2017; 2(1): 1-9
    » Abstract

  • Detection of equine herpesvirus type1and type 4 associated with abortion and respiratory manifestation in horses during 2016 in Cairo, Egypt
    Shimaa Mousleh Ghoniem, Ayman Hany El-Deeb, Mohamed Galal Aggour, Hussein Aly Hussein
    JVS. 2017; 2(1): 56-61
    » Abstract

  • ISOLATION AND IDENTIFICATION OF LUMPY SKIN DISEASE VIRUS IN CATTLE IN KALUBEYA GOVERNORATE
    Lamya Ateya Fathy Ateya, Ahmed Said Ahmed, Ayman Hamed Mansour, Ashraf Khames, Heba Ahmed Abdel Hady
    JVS. 2017; 1(Vol. 1, Jan. 2017): 12-19
    » Abstract

  • first isolation and identification of EHV-4 causing abortion in Egypt
    Ahmed Fawzy Afify, Basem M. Ahmed, Sayed Ahmed Salem, Ahmed Abd El-Ghani El-Sanousi, Mohamed Abd El-Hameed Shalaby
    JVS. 2017; 2(1): 92-101
    » Abstract

  • Most Downloaded
  • Standardization of Quality Control Protocol for Evaluation of Recombinant HVT-H5 Vaccine
    ola youssef mohamed, Ayman Hany Mahmoud, Samir Abdel- Moez Nasif, Ahmed Abdel El-Ghani El-Sanousi
    JVS. 2017; 1(Vol. 1, Jan. 2017): 27-39
    » Abstract

  • ISOLATION AND IDENTIFICATION OF LUMPY SKIN DISEASE VIRUS IN CATTLE IN KALUBEYA GOVERNORATE
    Lamya Ateya Fathy Ateya, Ahmed Said Ahmed, Ayman Hamed Mansour, Ashraf Khames, Heba Ahmed Abdel Hady
    JVS. 2017; 1(Vol. 1, Jan. 2017): 12-19
    » Abstract

  • Field application of trivalent foot and mouth disease vaccine adjuvanted with Zeolite.
    Hiam Fakhry Alhawary, Assem Mohamed abobaker, Sonia Ahmed Rizk, Akram Zakria Hegazi, Abobaker Ahmed Agoor
    JVS. 2017; 2(1): 32-41
    » Abstract

  • Detection of equine herpesvirus type1and type 4 associated with abortion and respiratory manifestation in horses during 2016 in Cairo, Egypt
    Shimaa Mousleh Ghoniem, Ayman Hany El-Deeb, Mohamed Galal Aggour, Hussein Aly Hussein
    JVS. 2017; 2(1): 56-61
    » Abstract

  • Identification and Molecular Characterization of a Phytoplasma Associated With Lemon Witches’-Broom in Egypt
    Rasha M. Mahrous, Ahmed K. El-Attar, Ahlam A. ALWatban, Sohair I. EL-Afifi, Nagwa M. Aref
    JVS. 2018; 3(1): 30-41
    » Abstract » doi: 283423

  • Very virulent IBDV strain Egypt/IBDV/Behera2011: Macroscopic and microscopic lesions accompanied with induced mortalities in SPF-chicks
    Reem A. Suliman, Basem M. Ahmed, Mounir M. El-Safty, Hussein Aly Hussein
    JVS. 2017; 2(1): 79-91
    » Abstract

  • Genetic characterization of rabbit hemorrhagic disease virus from naturally-infected rabbits in Sharkia governorate, Egypt
    Naglaa F.S. Awad, Gamelat K.F. Kotb
    JVS. 2018; 3(1): 10-19
    » Abstract » doi: 282147

  • Genetic and Antigenic Characterization of Infectious Bronchitis Virus Strains in Egypt
    Sara Mahmoud Ameen, Abdullah Selim, Ahmed El-Sanousi, Mohamed Shalaby
    JVS. 2017; 2(1): 42-55
    » Abstract

  • Molecular detection and phylogenetic analysis of sheep pox virus in El Menofiya Governorate.
    Ashraf Shabaan Khameis, lamya Fathi Atteya, Ayman Hmid Mansour, Heba Ahmad Abdelhady, Ashraf Ahmad Saad
    JVS. 2018; 3(1): 49-57
    » Abstract » doi: 276464

  • The co- infection of cucurbits with criniviruses and ipomoviruses: possible adverse effect on virus diagnosis and breeding for resistance.
    Aly M. Abdel-Salam
    JVS. 2017; 1(Vol. 1, Jan. 2017): 40-53
    » Abstract

  • Most Cited Articles