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A genotyping of a new avian infectious bronchitis virus isolated from chickens proventriculus in Egypt

Ehab M El-Nahas, Hemat S El-Sayed, Sawsan S El-Basuni, Gabr F El-Bagoury.

Abstract
A novel avian infectious bronchitis virus (IBV), Egypt/Qal/014p was isolated from 14-day-old broiler chickens with proventriculitis in Qaluobia province of Egypt in 2014. The isolate was identified by a real time RT-PCR targeting the nucleocapsid (N) gene. Sequence analysis of partial S1 gene of Egypt/Qal/014p had close relation with that of the Massachusetts prototypes. Comparisons nucleotide and amino acid sequence of partial S1 gene showed that the recent isolate had 98.2% and 95.2% nucleotide similarities and 96.8% and 91.4% amino acid similarities with the commonly used IBV vaccine Massachusetts strains H120 and M41 respectively. Egypt/Qal/014p had a unique amino acid substitution at residues 39 (Serine), 40 (Tyrosine), 41 (Lysine), 64 (Glutamate) and 69 (Valine) of the S1 proteins from H120 and M41.

The cross-neutralization test revealed antigenic relatedness of Egypt/Qal/014p with Massachusetts serotypes. The commercial H120 vaccine conferred partial protection against proventriculitis induced by Egypt/Qal/014p. In conclusion, IBV strains exhibiting proventriculitis were found co-circulating in broiler chickens in Egypt and optimum control of IBV in Egypt require preparation of vaccine from indigenous isolates beside periodic evaluation of cross-protective capabilities of such vaccine

Key words: Egypt/Qal/014p; Partial S1 gene analysis; Antigenic relatedness; Protection test


 
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How to Cite this Article
Pubmed Style

Ehab M El-Nahas, Hemat S El-Sayed, Sawsan S El-Basuni, Gabr F El-Bagoury. A genotyping of a new avian infectious bronchitis virus isolated from chickens proventriculus in Egypt. JVS. 2017; 1(Vol. 1, Jan. 2017): 54-66.


Web Style

Ehab M El-Nahas, Hemat S El-Sayed, Sawsan S El-Basuni, Gabr F El-Bagoury. A genotyping of a new avian infectious bronchitis virus isolated from chickens proventriculus in Egypt. http://www.j-virol-sci.com/?mno=246666 [Access: March 28, 2020].


AMA (American Medical Association) Style

Ehab M El-Nahas, Hemat S El-Sayed, Sawsan S El-Basuni, Gabr F El-Bagoury. A genotyping of a new avian infectious bronchitis virus isolated from chickens proventriculus in Egypt. JVS. 2017; 1(Vol. 1, Jan. 2017): 54-66.



Vancouver/ICMJE Style

Ehab M El-Nahas, Hemat S El-Sayed, Sawsan S El-Basuni, Gabr F El-Bagoury. A genotyping of a new avian infectious bronchitis virus isolated from chickens proventriculus in Egypt. JVS. (2017), [cited March 28, 2020]; 1(Vol. 1, Jan. 2017): 54-66.



Harvard Style

Ehab M El-Nahas, Hemat S El-Sayed, Sawsan S El-Basuni, Gabr F El-Bagoury (2017) A genotyping of a new avian infectious bronchitis virus isolated from chickens proventriculus in Egypt. JVS, 1 (Vol. 1, Jan. 2017), 54-66.



Turabian Style

Ehab M El-Nahas, Hemat S El-Sayed, Sawsan S El-Basuni, Gabr F El-Bagoury. 2017. A genotyping of a new avian infectious bronchitis virus isolated from chickens proventriculus in Egypt. Journal of Virological Sciences, 1 (Vol. 1, Jan. 2017), 54-66.



Chicago Style

Ehab M El-Nahas, Hemat S El-Sayed, Sawsan S El-Basuni, Gabr F El-Bagoury. "A genotyping of a new avian infectious bronchitis virus isolated from chickens proventriculus in Egypt." Journal of Virological Sciences 1 (2017), 54-66.



MLA (The Modern Language Association) Style

Ehab M El-Nahas, Hemat S El-Sayed, Sawsan S El-Basuni, Gabr F El-Bagoury. "A genotyping of a new avian infectious bronchitis virus isolated from chickens proventriculus in Egypt." Journal of Virological Sciences 1.Vol. 1, Jan. 2017 (2017), 54-66. Print.



APA (American Psychological Association) Style

Ehab M El-Nahas, Hemat S El-Sayed, Sawsan S El-Basuni, Gabr F El-Bagoury (2017) A genotyping of a new avian infectious bronchitis virus isolated from chickens proventriculus in Egypt. Journal of Virological Sciences, 1 (Vol. 1, Jan. 2017), 54-66.








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