ISSN 2636-2937
 

Original Research (Original Article) 


Standardization of Quality Control Protocol for Evaluation of Recombinant HVT-H5 Vaccine

ola youssef mohamed, Ayman Hany Mahmoud, Samir Abdel- Moez Nasif, Ahmed Abdel El-Ghani El-Sanousi.

Abstract
The present study was conducted to standardize a protocol for quality control and evaluation of the recombinant HVT-H5 vaccine. The protocol was designed for evaluation of H5 identity besides regular vaccine safety, purity and potency. The identity of H5 insert was evaluated using real-time PCR while the vaccine potency was evaluated using HVT virus titration, reduction of viral shedding during challenge test. Five vaccine batches were selected randomly and coded as A, B, C, D and E; these were examined and proved to be identical by quantitative real- time PCR in which the H5 gene titer was 7.364, 7.499, 7.767, 8.049, 8.000 logs 10 copies / ml respectively. The result of in- house ELISA was not significant for H5 gene detection and titration using polyclonal monospecific H5 serum. The HVT titer in CEF was 3045, 3200, 3400, 3750 & 4000 PFU/dose for batch A, B, C, D, and E respectively. The selected batches safety was satisfactory by 10 fold field doses injection in SPF chicks. The challenge test results revealed 80% protection for A, B and C batches while D and E batches gave 90% protection. The shedding of challenge virus was significantly low with mean of 2 logs 10 in the vaccinated group compared with control group.

Key words: Recombinant HVT-H5 Vaccine, evaluation, identity, potency, real-time PCR, Safety.


 
ARTICLE TOOLS
Abstract
PDF Fulltext
Print this article Print this Article
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by ola youssef mohamed
Articles by Ayman Hany Mahmoud
Articles by Samir Abdel- Moez Nasif
Articles by Ahmed Abdel El-Ghani El-Sanousi
on Google
on Google Scholar
Article Statistics
 Viewed: 2671
Downloaded: 2905
Cited: 0

How to Cite this Article
Pubmed Style

ola youssef mohamed, Ayman Hany Mahmoud, Samir Abdel- Moez Nasif, Ahmed Abdel El-Ghani El-Sanousi. Standardization of Quality Control Protocol for Evaluation of Recombinant HVT-H5 Vaccine. JVS. 2017; 1(Vol. 1, Jan. 2017): 27-39.


Web Style

ola youssef mohamed, Ayman Hany Mahmoud, Samir Abdel- Moez Nasif, Ahmed Abdel El-Ghani El-Sanousi. Standardization of Quality Control Protocol for Evaluation of Recombinant HVT-H5 Vaccine. http://www.j-virol-sci.com/?mno=245860 [Access: November 18, 2019].


AMA (American Medical Association) Style

ola youssef mohamed, Ayman Hany Mahmoud, Samir Abdel- Moez Nasif, Ahmed Abdel El-Ghani El-Sanousi. Standardization of Quality Control Protocol for Evaluation of Recombinant HVT-H5 Vaccine. JVS. 2017; 1(Vol. 1, Jan. 2017): 27-39.



Vancouver/ICMJE Style

ola youssef mohamed, Ayman Hany Mahmoud, Samir Abdel- Moez Nasif, Ahmed Abdel El-Ghani El-Sanousi. Standardization of Quality Control Protocol for Evaluation of Recombinant HVT-H5 Vaccine. JVS. (2017), [cited November 18, 2019]; 1(Vol. 1, Jan. 2017): 27-39.



Harvard Style

ola youssef mohamed, Ayman Hany Mahmoud, Samir Abdel- Moez Nasif, Ahmed Abdel El-Ghani El-Sanousi (2017) Standardization of Quality Control Protocol for Evaluation of Recombinant HVT-H5 Vaccine. JVS, 1 (Vol. 1, Jan. 2017), 27-39.



Turabian Style

ola youssef mohamed, Ayman Hany Mahmoud, Samir Abdel- Moez Nasif, Ahmed Abdel El-Ghani El-Sanousi. 2017. Standardization of Quality Control Protocol for Evaluation of Recombinant HVT-H5 Vaccine. Journal of Virological Sciences, 1 (Vol. 1, Jan. 2017), 27-39.



Chicago Style

ola youssef mohamed, Ayman Hany Mahmoud, Samir Abdel- Moez Nasif, Ahmed Abdel El-Ghani El-Sanousi. "Standardization of Quality Control Protocol for Evaluation of Recombinant HVT-H5 Vaccine." Journal of Virological Sciences 1 (2017), 27-39.



MLA (The Modern Language Association) Style

ola youssef mohamed, Ayman Hany Mahmoud, Samir Abdel- Moez Nasif, Ahmed Abdel El-Ghani El-Sanousi. "Standardization of Quality Control Protocol for Evaluation of Recombinant HVT-H5 Vaccine." Journal of Virological Sciences 1.Vol. 1, Jan. 2017 (2017), 27-39. Print.



APA (American Psychological Association) Style

ola youssef mohamed, Ayman Hany Mahmoud, Samir Abdel- Moez Nasif, Ahmed Abdel El-Ghani El-Sanousi (2017) Standardization of Quality Control Protocol for Evaluation of Recombinant HVT-H5 Vaccine. Journal of Virological Sciences, 1 (Vol. 1, Jan. 2017), 27-39.





Most Viewed Articles
  • Co-circulation of three different serotypes of FMD virus in Egypt
    Mahmoud talaat attia, Emad foad Elgendy, Momtaz Abdelhady Shahein, Samy Gomaa Kasem, madiha salah ibrahim
    JVS. 2017; 2(1): 102-113
    » Abstract

  • Isolation of H5 Highly Pathogenic Avian Influenza Virus from Cattle Egret (Bubulcus ibis) near Affected Broiler Chicken Flocks in Egypt
    Moustafa M. El-Shazly, Basem M. Ahmed, Ahmed A. El-Sanousi, Youssef I. Youssef
    JVS. 2017; 2(1): 62-70
    » Abstract

  • Identification and Characterization of Soybean vein necrosis virus (SVNV): A newly Isolated Thrips-Borne Tospovirus in Egypt
    Abeer Salah Abd El-Wahab, Manal Ali El-Shazly
    JVS. 2017; 1(Vol. 1, Jan. 2017): 76-90
    » Abstract

  • Field application of trivalent foot and mouth disease vaccine adjuvanted with Zeolite.
    Hiam Fakhry Alhawary, Assem Mohamed abobaker, Sonia Ahmed Rizk, Akram Zakria Hegazi, Abobaker Ahmed Agoor
    JVS. 2017; 2(1): 32-41
    » Abstract

  • Elimination of Alfalfa mosaic virus (AMV) from infected potato leaves (Solanum tuberosum) cv. Ditta by embryonic calli
    Maha Ahmed Maher El-Abhar, Moustafa Ahmed Salama El-Kady, Khaled Mohamed Ghanem, Hussieny Abd-Elhak Bosila
    JVS. 2017; 1(Vol. 1, Jan. 2017): 100-113
    » Abstract

  • Herpes Simplex virus type 1 persistence in water from different sources
    Dina Nadeem Abd-Elshafy, Mahmoud Mohamed Bahgat
    JVS. 2017; 2(1): 114-123
    » Abstract

  • ISOLATION AND IDENTIFICATION OF LUMPY SKIN DISEASE VIRUS IN CATTLE IN KALUBEYA GOVERNORATE
    Lamya Ateya Fathy Ateya, Ahmed Said Ahmed, Ayman Hamed Mansour, Ashraf Khames, Heba Ahmed Abdel Hady
    JVS. 2017; 1(Vol. 1, Jan. 2017): 12-19
    » Abstract

  • Detection of equine herpesvirus type1and type 4 associated with abortion and respiratory manifestation in horses during 2016 in Cairo, Egypt
    Shimaa Mousleh Ghoniem, Ayman Hany El-Deeb, Mohamed Galal Aggour, Hussein Aly Hussein
    JVS. 2017; 2(1): 56-61
    » Abstract

  • Prevalence of equine herpes viruses 1 and 4 in Arabian horses population in Egypt
    Soha Ibrahim Mohamed, Mohamed Abd-Elhameed Shalaby, Ayman Hany El Deeb, Sayed Ahmed Salem
    JVS. 2017; 2(1): 1-9
    » Abstract

  • Very virulent IBDV strain Egypt/IBDV/Behera2011: Macroscopic and microscopic lesions accompanied with induced mortalities in SPF-chicks
    Reem A. Suliman, Basem M. Ahmed, Mounir M. El-Safty, Hussein Aly Hussein
    JVS. 2017; 2(1): 79-91
    » Abstract

  • Most Downloaded
  • Standardization of Quality Control Protocol for Evaluation of Recombinant HVT-H5 Vaccine
    ola youssef mohamed, Ayman Hany Mahmoud, Samir Abdel- Moez Nasif, Ahmed Abdel El-Ghani El-Sanousi
    JVS. 2017; 1(Vol. 1, Jan. 2017): 27-39
    » Abstract

  • ISOLATION AND IDENTIFICATION OF LUMPY SKIN DISEASE VIRUS IN CATTLE IN KALUBEYA GOVERNORATE
    Lamya Ateya Fathy Ateya, Ahmed Said Ahmed, Ayman Hamed Mansour, Ashraf Khames, Heba Ahmed Abdel Hady
    JVS. 2017; 1(Vol. 1, Jan. 2017): 12-19
    » Abstract

  • Field application of trivalent foot and mouth disease vaccine adjuvanted with Zeolite.
    Hiam Fakhry Alhawary, Assem Mohamed abobaker, Sonia Ahmed Rizk, Akram Zakria Hegazi, Abobaker Ahmed Agoor
    JVS. 2017; 2(1): 32-41
    » Abstract

  • Very virulent IBDV strain Egypt/IBDV/Behera2011: Macroscopic and microscopic lesions accompanied with induced mortalities in SPF-chicks
    Reem A. Suliman, Basem M. Ahmed, Mounir M. El-Safty, Hussein Aly Hussein
    JVS. 2017; 2(1): 79-91
    » Abstract

  • Detection of equine herpesvirus type1and type 4 associated with abortion and respiratory manifestation in horses during 2016 in Cairo, Egypt
    Shimaa Mousleh Ghoniem, Ayman Hany El-Deeb, Mohamed Galal Aggour, Hussein Aly Hussein
    JVS. 2017; 2(1): 56-61
    » Abstract

  • Identification and Molecular Characterization of a Phytoplasma Associated With Lemon Witches’-Broom in Egypt
    Rasha M. Mahrous, Ahmed K. El-Attar, Ahlam A. ALWatban, Sohair I. EL-Afifi, Nagwa M. Aref
    JVS. 2018; 3(1): 30-41
    » Abstract » doi: 283423

  • Genetic and Antigenic Characterization of Infectious Bronchitis Virus Strains in Egypt
    Sara Mahmoud Ameen, Abdullah Selim, Ahmed El-Sanousi, Mohamed Shalaby
    JVS. 2017; 2(1): 42-55
    » Abstract

  • Genetic characterization of rabbit hemorrhagic disease virus from naturally-infected rabbits in Sharkia governorate, Egypt
    Naglaa F.S. Awad, Gamelat K.F. Kotb
    JVS. 2018; 3(1): 10-19
    » Abstract » doi: 282147

  • Molecular characterization of foot and mouth disease virus (O-EA3) isolated during 2016 outbreak in Egypt
    Muhammad Gamal Khodary, Ayman Hanea El-Deeb, mohamed maged Emara, Othman El mahdy Othman, Hussein Ali Hussein
    JVS. 2018; 3(1): 1-9
    » Abstract » doi: 286803

  • Molecular detection and phylogenetic analysis of sheep pox virus in El Menofiya Governorate.
    Ashraf Shabaan Khameis, lamya Fathi Atteya, Ayman Hmid Mansour, Heba Ahmad Abdelhady, Ashraf Ahmad Saad
    JVS. 2018; 3(1): 49-57
    » Abstract » doi: 276464

  • Most Cited Articles