Open Access and Peer-reviewed
Home Journal Issues Guide for Authors Editorial Board Aims & Scope About Journal News & Announcements


Original Article 


ISOLATION AND IDENTIFICATION OF LUMPY SKIN DISEASE VIRUS IN CATTLE IN KALUBEYA GOVERNORATE

Lamya Ateya Fathy Ateya, Ahmed Said Ahmed, Ayman Hamed Mansour, Ashraf Khames, Heba Ahmed Abdel Hady.

Abstract
Lumpy skin disease virus (LSDV) is an infectious viral disease which is an important serious skin disease of cattle. LSDV was isolated from skin biopsies collected from cattle which are clinically infected at Kaluobia governorate. A total 23 skin biopsies were used for virus isolatation on chorio-allantoic membrane (CAM) of embryonated chicken eggs SPF at 9-11 day which showed pock lesions and CPE on MDBK cell culture. Isolated virus was identified by indirect fluorescent antibody technique (IFAT) using specific hyper immune serum against Lumpy Skin Disease Virus. Further identifications were carried out by polymerase chain reaction (PCR).15 samples were +ve for LSDV by conventional PCR.The results showed that 11/23 biopsies were positive by IFAT. Molecular identification of LSD virus by using RT-PCR, revealed positive for amplification of bands at the predicted molecular size (1926bp). Neutralizing antibodies against LSDV were (55) out of total 100 serum samples by serum neutralization test. Selection and processing of clinical specimens, viral isolation and PCR assay applied, for LSDV are much sensitive and rapid diagnostic tool of LSD reflecting their importance in controlling the rapid spread of disease in Egypt.

Key words: LSDV, CAM, MDBK, IFAT, PCR.


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Lamya Ateya Fathy Ateya
Articles by Ahmed Said Ahmed
Articles by Ayman Hamed Mansour
Articles by Ashraf Khames
Articles by Heba Ahmed Abdel Hady
on Google
on Google Scholar
Article Statistics
 Viewed: 3181
Downloaded: 941


How to Cite this Article
Pubmed Style

Lamya Ateya Fathy Ateya, Ahmed Said Ahmed, Ayman Hamed Mansour, Ashraf Khames, Heba Ahmed Abdel Hady. ISOLATION AND IDENTIFICATION OF LUMPY SKIN DISEASE VIRUS IN CATTLE IN KALUBEYA GOVERNORATE. JVS. 2017; 1(Vol. 1, Jan. 2017): 12-19.


Web Style

Lamya Ateya Fathy Ateya, Ahmed Said Ahmed, Ayman Hamed Mansour, Ashraf Khames, Heba Ahmed Abdel Hady. ISOLATION AND IDENTIFICATION OF LUMPY SKIN DISEASE VIRUS IN CATTLE IN KALUBEYA GOVERNORATE. http://www.j-virol-sci.com/?mno=245655 [Access: January 19, 2020].


AMA (American Medical Association) Style

Lamya Ateya Fathy Ateya, Ahmed Said Ahmed, Ayman Hamed Mansour, Ashraf Khames, Heba Ahmed Abdel Hady. ISOLATION AND IDENTIFICATION OF LUMPY SKIN DISEASE VIRUS IN CATTLE IN KALUBEYA GOVERNORATE. JVS. 2017; 1(Vol. 1, Jan. 2017): 12-19.



Vancouver/ICMJE Style

Lamya Ateya Fathy Ateya, Ahmed Said Ahmed, Ayman Hamed Mansour, Ashraf Khames, Heba Ahmed Abdel Hady. ISOLATION AND IDENTIFICATION OF LUMPY SKIN DISEASE VIRUS IN CATTLE IN KALUBEYA GOVERNORATE. JVS. (2017), [cited January 19, 2020]; 1(Vol. 1, Jan. 2017): 12-19.



Harvard Style

Lamya Ateya Fathy Ateya, Ahmed Said Ahmed, Ayman Hamed Mansour, Ashraf Khames, Heba Ahmed Abdel Hady (2017) ISOLATION AND IDENTIFICATION OF LUMPY SKIN DISEASE VIRUS IN CATTLE IN KALUBEYA GOVERNORATE. JVS, 1 (Vol. 1, Jan. 2017), 12-19.



Turabian Style

Lamya Ateya Fathy Ateya, Ahmed Said Ahmed, Ayman Hamed Mansour, Ashraf Khames, Heba Ahmed Abdel Hady. 2017. ISOLATION AND IDENTIFICATION OF LUMPY SKIN DISEASE VIRUS IN CATTLE IN KALUBEYA GOVERNORATE. Journal of Virological Sciences, 1 (Vol. 1, Jan. 2017), 12-19.



Chicago Style

Lamya Ateya Fathy Ateya, Ahmed Said Ahmed, Ayman Hamed Mansour, Ashraf Khames, Heba Ahmed Abdel Hady. "ISOLATION AND IDENTIFICATION OF LUMPY SKIN DISEASE VIRUS IN CATTLE IN KALUBEYA GOVERNORATE." Journal of Virological Sciences 1 (2017), 12-19.



MLA (The Modern Language Association) Style

Lamya Ateya Fathy Ateya, Ahmed Said Ahmed, Ayman Hamed Mansour, Ashraf Khames, Heba Ahmed Abdel Hady. "ISOLATION AND IDENTIFICATION OF LUMPY SKIN DISEASE VIRUS IN CATTLE IN KALUBEYA GOVERNORATE." Journal of Virological Sciences 1.Vol. 1, Jan. 2017 (2017), 12-19. Print.



APA (American Psychological Association) Style

Lamya Ateya Fathy Ateya, Ahmed Said Ahmed, Ayman Hamed Mansour, Ashraf Khames, Heba Ahmed Abdel Hady (2017) ISOLATION AND IDENTIFICATION OF LUMPY SKIN DISEASE VIRUS IN CATTLE IN KALUBEYA GOVERNORATE. Journal of Virological Sciences, 1 (Vol. 1, Jan. 2017), 12-19.








AUTHOR LOGIN

REVIEWER LOGIN

Indexed
&
Abstracted



ABOUT JOURNAL
POLICIES
STATEMENTS

This is an open access journal which means that all content is freely available without charge to the user or his/her institution. Users are allowed to read, download, copy, distribute, print, search, or link to the full texts of the articles in this journal without asking prior permission from the publisher or the author. This is in accordance with the Budapest Open Access Initiative (BOAI) definition of open access.

The articles in Journal of Virological Sciences are open access articles licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc-sa/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.