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Original Research (Original Article) 
JVS. 2019; 5(1): 11-21
doi: 1402


Sequence Analysis of Seven Equine Herpes Type 1 Viruses Circulating In Non- vaccinated Arabian and Foreign Horses In Egypt

Nagwa Khaled Meselhy, Mohamed Abdelhamid Abo El-khair, Basem M. Ahmed, Sherif Abdelghaffar El-Soally, Abdelhamid Mohamed Fares, Mohamed Abualez Nayel, Hussein Ali Hussein.

Abstract
Background: Equine herpes virus 1 (EHV-1) has a significant economic impact on the equine industry. It is a highly contagious pathogen, mainly transmitted by inhalation of aerosols of virus-laden respiratory secretions especially with the high population during equestrian events. Reactivation of virus in non-symptomatic, latently (silently) infected horses is the main cause of circulation of such virus in horses.
Aim: Molecular characterization of the circulating strains of Equine herpes viruses in the Egyptian equestrian clubs.
Methods: In the present study, molecular characterization of circulating EHV-1 viruses among horses of equestrian clubs in Egypt was carried out. Sixty-two samples of whole blood with anticoagulant were collected and screened for EHV-1 using nested PCR that amplifies the conserved fragment of glycoprotein B gene (ORF 33), followed by sequencing and phylogenetic analysis.
Results: The study revealed that 19% of our samples were positive to EHV-1 and phylogenetic analysis showed that the Egyptian EHV-1 isolates were more than 99% similarity to European abortogenic isolates (EHV-1 strains: Army 183 and Suffolk/48/2013). Indeed, the analysis reports that these viruses are circulating in both Arabian and foreign horses in Egypt.
Conclusion: The nested PCR is an appropriate technique for the detection of EHV-1 present in blood of a latently, or silently infected horses. The application of this nested PCR is considered to be a rapid diagnostic tool for use in veterinary laboratories. Thus it will enhance the detection and characterization of the circulating EHV-1 in Egypt. Control strategy for EHV-1 viruses in vaccinated and non-vaccinated animals needs to be addressed.

Key words: Nested PCR, equine herpes virus type 1 (EHV-1), glycoprotein B gene (gB, ORF 33), horses, Egypt


 
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How to Cite this Article
Pubmed Style

Meselhy NK, El-khair MAA, Ahmed BM, El-Soally SA, Fares AM, Nayel MA, Hussein HA, . Sequence Analysis of Seven Equine Herpes Type 1 Viruses Circulating In Non- vaccinated Arabian and Foreign Horses In Egypt. JVS. 2019; 5(1): 11-21. doi:1402


Web Style

Meselhy NK, El-khair MAA, Ahmed BM, El-Soally SA, Fares AM, Nayel MA, Hussein HA, . Sequence Analysis of Seven Equine Herpes Type 1 Viruses Circulating In Non- vaccinated Arabian and Foreign Horses In Egypt. http://www.j-virol-sci.com/?mno=1402 [Access: March 28, 2020]. doi:1402


AMA (American Medical Association) Style

Meselhy NK, El-khair MAA, Ahmed BM, El-Soally SA, Fares AM, Nayel MA, Hussein HA, . Sequence Analysis of Seven Equine Herpes Type 1 Viruses Circulating In Non- vaccinated Arabian and Foreign Horses In Egypt. JVS. 2019; 5(1): 11-21. doi:1402



Vancouver/ICMJE Style

Meselhy NK, El-khair MAA, Ahmed BM, El-Soally SA, Fares AM, Nayel MA, Hussein HA, . Sequence Analysis of Seven Equine Herpes Type 1 Viruses Circulating In Non- vaccinated Arabian and Foreign Horses In Egypt. JVS. (2019), [cited March 28, 2020]; 5(1): 11-21. doi:1402



Harvard Style

Meselhy, N. K., El-khair, M. A. A., Ahmed, B. M., El-Soally, S. A., Fares, A. M., Nayel, M. A., Hussein, H. A. & (2019) Sequence Analysis of Seven Equine Herpes Type 1 Viruses Circulating In Non- vaccinated Arabian and Foreign Horses In Egypt. JVS, 5 (1), 11-21. doi:1402



Turabian Style

Meselhy, Nagwa Khaled, Mohamed Abdelhamid Abo El-khair, Basem M. Ahmed, Sherif Abdelghaffar El-Soally, Abdelhamid Mohamed Fares, Mohamed Abualez Nayel, Hussein Ali Hussein, and . 2019. Sequence Analysis of Seven Equine Herpes Type 1 Viruses Circulating In Non- vaccinated Arabian and Foreign Horses In Egypt. Journal of Virological Sciences, 5 (1), 11-21. doi:1402



Chicago Style

Meselhy, Nagwa Khaled, Mohamed Abdelhamid Abo El-khair, Basem M. Ahmed, Sherif Abdelghaffar El-Soally, Abdelhamid Mohamed Fares, Mohamed Abualez Nayel, Hussein Ali Hussein, and . "Sequence Analysis of Seven Equine Herpes Type 1 Viruses Circulating In Non- vaccinated Arabian and Foreign Horses In Egypt." Journal of Virological Sciences 5 (2019), 11-21. doi:1402



MLA (The Modern Language Association) Style

Meselhy, Nagwa Khaled, Mohamed Abdelhamid Abo El-khair, Basem M. Ahmed, Sherif Abdelghaffar El-Soally, Abdelhamid Mohamed Fares, Mohamed Abualez Nayel, Hussein Ali Hussein, and . "Sequence Analysis of Seven Equine Herpes Type 1 Viruses Circulating In Non- vaccinated Arabian and Foreign Horses In Egypt." Journal of Virological Sciences 5.1 (2019), 11-21. Print. doi:1402



APA (American Psychological Association) Style

Meselhy, N. K., El-khair, M. A. A., Ahmed, B. M., El-Soally, S. A., Fares, A. M., Nayel, M. A., Hussein, H. A. & (2019) Sequence Analysis of Seven Equine Herpes Type 1 Viruses Circulating In Non- vaccinated Arabian and Foreign Horses In Egypt. Journal of Virological Sciences, 5 (1), 11-21. doi:1402








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